Screening and identification of BSA bound ligands from Puerariae lobata flower by BSA functionalized Fe3O4 magnetic nanoparticles coupled with HPLC-MS/MS
Screening and identification of BSA bound ligands from Puerariae lobata flower by BSA functionalized Fe3O4 magnetic nanoparticles coupled with HPLC-MS/MS
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BSA 功能化 Fe3O4 磁性纳米颗粒与 HPLC-MS/MS 联用筛选和鉴定葛花中 BSA 结合配体
DOI：
10.1016/j.jchromb.2012.01.008
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发表时间：
2012-03-01
影响因子：
3
通讯作者：
Shi, Shuyun
中科院分区：
文献类型：
--
作者：
Liu, Liangliang;Ma, Yongjian;Shi, Shuyun
葛花中国具有悠久的治疗酒精中毒的历史，其主要药理活性成分为一系列异黄酮类。本研究采用牛血清白蛋白(BSA)功能化的氧化铁磁性纳米颗粒(Fe3O4 MNPs)与高效液相色谱-串联质谱仪(HPLC-MS/MS)相结合的方法，对黄花丹参醇提物中的活性成分进行了筛选和鉴定。分离鉴定了13个活性异黄酮类化合物，分别为甘油素(1)、山茶素(2)、大豆苷(3)、3‘-甲氧基大豆苷元(4)、芒柄花素(5)、3’-羟基大豆苷元(6)、山茶素(7)、木犀草素A(8)、李素(9)、染料木素(10)、3‘-甲氧基大豆苷元(11)、淫羊藿酮(12)和5，7-二羟基-3’，4‘-亚甲基二氧基异黄酮(13)。化合物4、6、9、11和13为首次从该植物中分离鉴定。此外，还在线评估了各结合配体的活性。结果表明，化合物与牛血清白蛋白的结合亲和力高度依赖于化学结构，B环上的甲氧基化和羟基化可以提高其活性。该方法可广泛应用于复杂混合物中活性物质的快速筛选和鉴定。(C)2012爱思唯尔B.V.保留所有权利。
Puerariae lobata flower (Willdenow) has a long history used to treat alcoholic intoxication in China, which contains a series of isoflavones as its chief pharmacologically active constituents. In this study, bovine serum albumin (BSA) functionalized iron oxide magnetic nanoparticles (Fe3O4 MNPs) coupled with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were used to screen and identify active compounds from ethanolic extract of P. lobata flower. Thirteen active isoflavones were screened and identified as glycitin (1), tectoridin (2), daidzin (3), 3'-methoxy daidzin (4), ononin (5), 3'-hydroxyl daidzein (6), tectorigenin (7), biochanin A (8), prunetin (9), genistein (10), 3'-methoxy daidzein (11), irisolidone (12) and 5,7-dihydroxy-3',4'-methylenedioxyisoflavone (13), while compounds 4, 6, 9, 11 and 13 were identified from this plant for the first time. Furthermore, the activity of each bound ligand was evaluated on-line. The results indicated that the binding affinities of compounds with BSA were highly dependent on chemical structures and the methoxylation and hydroxylation on B-ring could improve the activity. The effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures. (c) 2012 Elsevier B.V. All rights reserved.