PRESENCE AND SYNTHESIS OF INHIBIN SUBUNITS IN HUMAN DECIDUA
PRESENCE AND SYNTHESIS OF INHIBIN SUBUNITS IN HUMAN DECIDUA
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DOI：
10.1210/jcem-71-2-487
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发表时间：
1990-08-01
影响因子：
5.8
通讯作者：
MEUNIER, H
中科院分区：
文献类型：
--
作者：
PETRAGLIA, F;CALZA, L;MEUNIER, H
越来越多的研究表明，人蜕膜是一种与妊娠有关的组织，能够产生和代谢激素。本研究的目的是评估抑制素亚基在人蜕膜中的可能存在。在妊娠早期（8周）、中期（18周）和足月（40周）的孕妇中收集组织样本。免疫组化数据采用兔抗猪α、β的亲和纯化多克隆抗血清获得。A，或者。B亚基。利用cDNA探针编码每个亚基对应的序列，通过Northern blot分析评估各自抑制素亚基的水平。目前的研究结果表明，人蜕膜含有和合成抑制素。A和。B亚基。免疫组化数据显示，蜕膜细胞均有抑制素。和度量。B抗血清，显示类似的定位。另一方面，细胞被抑制素染色。抗血清稀疏，其分布模式与α染色细胞不同。或度量。抗血清。第一个ingbin。alpha。和度量。B亚基mRNA均在妊娠早期表达，且mRNA水平呈妊娠期增高。度量。一个亚基mRNA在足月时表达水平非常低，在妊娠早期无法检测到。目前的数据显示，人蜕膜积极产生抑制素亚单位与妊娠相关的轮廓。结果提示蜕膜可能是妊娠期抑制素相关蛋白的另一个来源，并强调了人蜕膜的内分泌能力。
A growing number of studies provided the evidence that human decidua is a pregnancy-related tissue capable of hormone production and metabolism. The aim of the present study was to evaluate the possible presence of inhibin subunits in human decidua. Tissue samples were collected in pregnant women during the first (8 weeks) and second trimester (18 weeks) of gestation and at term (40 weeks). Immunohistochemical data were obtained using affinity purified polyclonal antisera raised in rabbit against porcine .alpha.,.beta.A, or .beta.B subunits. Levels of the respective inhibin subunits were evaluated by Northern blot analysis using cDNA probes encoding sequences corresponding to each subunit. The present results indicated that human decidua contains and synthesizes inhibin .alpha.,.beta.A, and .beta.B subunits. The immunohistochemical data showed that decidual cells were stained with both inhibin .alpha. and .beta.B antisera, showing a similar localization. On the other hand, cells stained with inhibin .beta.A antisera were sparse and followed a distribution pattern different from that of cells stained with .alpha. or .beta.B antisera. The first ingibin .alpha. and .beta.B subunit mRNAs were both expressed in first trimester of pregnancy, and those mRNA levels showed a gestational related increase. The .beta.A subunit mRNA was expressed at very low levels at term and could not be detected earlier during pregnancy. The present data showed that human decidua actively produces inhibin subunits with a gestational-related profile. The results suggest that decidua may be a further source of inhibin-related proteins during pregnancy and emphasize the endocrine competence of human decidua.