Cloning of a 9-cis-epoxycarotenoid dioxygenase gene and the responses of Caragana korshinskii to a variety of abiotic stresses.
Cloning of a 9-cis-epoxycarotenoid dioxygenase gene and the responses of Caragana korshinskii to a variety of abiotic stresses.
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DOI：
10.1266/ggs.84.397
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发表时间：
2009-12
影响因子：
1.1
通讯作者：
Xuemin Wang;Zan Wang;Jie Dong;Meizhen Wang;Hong-wen Gao
中科院分区：
文献类型：
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作者：
Xuemin Wang;Zan Wang;Jie Dong;Meizhen Wang;Hong-wen Gao
脱落酸（阿坝）调节植物对各种环境胁迫的适应性反应。9-顺式环氧类胡萝卜素双加氧酶（NCED）催化的顺式环氧类胡萝卜素氧化裂解是高等植物阿坝生物合成的主要调控步骤。利用RACE技术从柠条锦鸡儿叶片中克隆了NCED基因的全长cDNA。CkNCED 1全长2442 bp，开放阅读框1818 bp，编码605个氨基酸。CkNCED 1蛋白的氨基酸序列与其他NCED蛋白具有高度的同源性。Southern杂交分析表明，CkNCED 1基因在C. Korshinskii。当C. korshinskii植物暴露于水分亏缺，阿坝积累，其次是CkNCED 1 mRNA在叶和茎的大幅增加，但只有适度的增加，在根。相反，胁迫叶片的复水引起CkNCED 1 mRNA和阿坝水平的快速下降。RT-PCR和荧光定量PCR分析表明，盐胁迫可诱导CkNCED 1在盐胁迫下迅速在黄瓜叶片和根中大量表达。korshinskii，以及阿坝积累。低温胁迫和外源阿坝的施用也诱导了CkNCED 1的表达和阿坝的积累。综上所述，这些结果表明CkNCED 1可能在C. Korshinskii。
Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses. Oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is the main regulatory step in the biosynthesis of ABA in higher plants. Using RACE technology, a full-length cDNA-encoding NCED gene was isolated and characterized from the leaves of Caragana korshinskii (Peashrub). The 2442-bp full-length CkNCED1 had a 1818-bp ORF, which encodes a peptide of 605 amino acids. The deduced amino acid sequence of CkNCED1 protein shared high identity with other NCEDs. Southern blot analysis revealed that the gene CkNCED1 was a single copy in the genome of C. korshinskii. When C. korshinskii plants were exposed to a water deficit, ABA accumulation was followed by large increases in CkNCED1 mRNA in leaves and stems, but only a moderate increase in the roots. Conversely, rehydration of stressed leaves caused a rapid decrease in CkNCED1 mRNA and ABA levels. RT-PCR and Quantitative real-time PCR analysis showed that salt stress rapidly induced the strong expression of CkNCED1 in leaves and roots of C. korshinskii, as well as ABA accumulation. The expression of CkNCED1 and ABA accumulation was also induced by cold stress and the application of exogenous ABA. Taken together, these results suggest that CkNCED1 likely plays a primary role in the biosynthesis of ABA in C. korshinskii.