Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma
Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma
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从液化人精浆中鉴定新型精蛋白 I 衍生抗菌肽
DOI：
10.1016/j.peptides.2008.01.009
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发表时间：
2008-04-01
期刊：
影响因子：
3
通讯作者：
Zhang, Yun
中科院分区：
文献类型：
--
作者：
Zhao, Hui;Lee, Wen-Hui;Zhang, Yun
Semenogelin I （SgI）是人类精浆中最丰富的蛋白质之一。SgI在精子凝固和精子固定中起关键作用。此外，SgI和/或其蛋白水解片段参与调节精子运动、获能和抑制素样活性。然而，对sgi衍生肽的抗菌活性知之甚少。采用离子交换、凝胶过滤和高效液相色谱相结合的方法，从40例健康供者的液化人精浆中分离并鉴定了多肽。n端氨基酸测序和快速原子轰击质谱分析结果显示，4个分离肽均来源于sgi，分别为SgI-29 （8S-113）、SgI-46（85-130）、SgI-47（85-131）和SgI-52（85-136）。有趣的是，SgI-29、SgI-46和SgI-47是新发现的sgi衍生肽。抑菌活性试验结果表明，合成的SgI-29对多种细菌均有较强的抑菌活性。我们的研究结果表明，SgI可以被消化成小片段，如新鉴定的SgI-29、SgI-46和SgI-47，并且可能具有多种功能。(C) 2008爱思唯尔公司版权所有。
Semenogelin I (SgI) is one of the most abundant proteins in human seminal plasma. SgI plays a key role in sperm coagulation and spermatozoon immobilization. in addition, SgI and/or its proteolytic fragments are involved in regulating spermatozoon motility, capacitation and inhibin-like activity. However, little is known about the antibacterial activity of SgI-derived peptides. By a combination of ion-exchange, gel filtration and high-performance liquid chromatography, peptides from liquefied human seminal plasma from 40 healthy donors were isolated and characterized. N-terminal amino-acid sequencing and fast atom bombardment mass spectrometry revealed that four isolated peptides were SgI-derived, namely SgI-29 (8S-113), SgI-46 (85-130), SgI-47 (85-131) and SgI-52 (85-136). Interestingly, SgI-29, SgI-46 and SgI-47 are newly identified SgI-derived peptides. Antimicrobial activity assay results indicated that synthesized SgI-29 had strong antibacterial activity toward various bacterial strains. Our results indicate that SgI can be digested into small fragments like newly identified SgI-29, SgI-46 and SgI-47 and may have diversified functions. (C) 2008 Elsevier Inc. All rights reserved.