Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo.
Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo.
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DOI：
10.1242/dev.156406
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发表时间：
2017-10-15
期刊：
影响因子：
--
通讯作者：
Plusa B
中科院分区：
文献类型：
--
作者：
Piliszek A;Madeja ZE;Plusa B
外胚层 (EPI)（所有胚胎谱系的创始系）和胚胎外支持组织的形成是哺乳动物发育的关键事件之一。早期哺乳动物发育的流行模型几乎完全基于小鼠。在这里，我们对兔子植入前发育过程中的 EPI 和胚胎外原始内胚层 (PrE) 形成进行了全面的分阶段分析。尽管我们观察到兔子胚胎与小鼠胚胎有几个共同特征，包括与阶段相关的谱系规范起始，但我们的结果表明哺乳动物之间的谱系规范存在一些关键差异。与目前的观点相反，我们的数据表明，GATA6 和 NANOG 的相互抑制对于哺乳动物中 PrE 与 EPI 规范的初始阶段并不重要。此外，我们的结果还深入了解了小鼠和其他哺乳动物之间观察到的与 PrE 与 EPI 规范中 FGF/ERK 信号传导作用相关的差异。 对兔子植入前发育的全面分析揭示了兔子和小鼠之间的关键差异，兔子的谱系规范的某些方面更接近人类和灵长类动物胚胎。
Formation of epiblast (EPI) – the founder line of all embryonic lineages – and extra-embryonic supportive tissues is one of the key events in mammalian development. The prevailing model of early mammalian development is based almost exclusively on the mouse. Here, we provide a comprehensive, stage-by-stage analysis of EPI and extra-embryonic primitive endoderm (PrE) formation during preimplantation development of the rabbit. Although we observed that rabbit embryos have several features in common with mouse embryos, including a stage-related initiation of lineage specification, our results demonstrate the existence of some key differences in lineage specification among mammals. Contrary to the current view, our data suggest that reciprocal repression of GATA6 and NANOG is not fundamental for the initial stages of PrE versus EPI specification in mammals. Furthermore, our results provide insight into the observed discrepancies relating to the role of FGF/ERK signalling in PrE versus EPI specification between mouse and other mammals. A comprehensive analysis of rabbit preimplantation development reveals key differences between rabbit and mouse, with some aspects of lineage specification in rabbit more closely resembling that of human and primate embryos.
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