The molecular weight and packaging of dsRNAs in the mycovirus from Ustilago maydis killer strains.
The molecular weight and packaging of dsRNAs in the mycovirus from Ustilago maydis killer strains.
复制标题
玉米黑粉菌杀手菌株真菌病毒中 dsRNA 的分子量和包装。
DOI：
10.1016/0042-6822(81)90177-x
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发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
R. Steinlauf
中科院分区：
文献类型：
--
作者：
R. F. Bozarth;Y. Koltint;M. Weissman;R. Parker;R. E. Dalton;R. Steinlauf
梅氏黑粉菌的真菌病毒是等轴的，直径为43 nm，并含有几个dsRNA片段，根据其相对大小命名为重链（H）、中链（M）和轻链（L）。为了确定每个病毒体的dsRNA片段的数量，分离主要的沉降和密度组分，从流体动力学性质确定其分子量，并通过电子显微镜和/或聚丙烯酰胺凝胶电泳确定其dsRNA含量。2.9、3.1和4.2 × 106道尔顿的H dsRNA片段分别以1.383、1.394和1.418 g/cm ~ 8的浓度存在于CsCl中。P1菌株含有3.1和4.2 × 106-道尔顿片段，3103菌株含有2.9和4.2 × 106-道尔顿片段。T-4株含有3.1 × 106-道尔顿的H片段和0.67和0.60 × 106道尔顿的两个M片段。H片段分别在病毒粒子中以1.394 g/cm 8条带化，而M片段在病毒粒子中以1、2或3组以1.314、1.341和1.370 g/cm 8条带化。空衣壳（pCsCl = 1.278 g/cm 8）和含有3.1 × 106-道尔顿dsRNA片段的衣壳（pCsCl = 1.394 g/cm 8）的分子量计算为9.8和13.0 × 106道尔顿。对CsCl中在其他密度下条带化的组分的分析与以下假设一致：条带模式是9.8 × 106道尔顿衣壳中有限数量dsRNA片段的双链化结果。尽管在单个病毒体中存在1 - 3个M dsRNA片段，但没有检测到每个病毒体具有超过1个H dsRNA片段的颗粒。
The mycoviruses ofUstilago maydiskiller strains are isometric, 43 nm in diameter, and contain several dsRNA segments designated heavy (H), medium (M), and light (L) according to their relative size. To determine the number of dsRNA segments per virion, major sedimenting and density components were isolated, their molecular weights determined from hydrodynamic properties, and their dsRNA contents determined by electron microscopy and/or polyacrylamide gel electrophoresis. The H dsRNA segments of 2.9, 3.1, and 4.2 × 106daltons are separately encapsidated in isometric capsids that band in CsCI at 1.383, 1.394, and 1.418 g/cm8, respectively. The P1 strain contains the 3.1 and 4.2 × 106-dalton segments, and the 3103 strain contains the 2.9 and 4.2 × 106-dalton segments. The T-4 strain contains the 3.1 x 106-dalton H segment and two M segments of 0.67 and 0.60 × 106daltons. The H segments are separately encapsidated in virions which banded at 1.394 g/cm8, whereas the M segments are encapsidated in sets of one, two, or three in virions which banded at 1.314, 1.341, and 1.370 g/cm8. Molecular weights of 9.8 and 13.0 × 106 daltons were calculated for empty capsids (pCsCl = 1.278 g/cm8) and capsids containing the 3.1 × 106-dalton dsRNA segments (pCsCl = 1.394 g/cm8). Analysis of components that banded at other densities in CsCl were consistent with the hypothesis that the banding pattern is the result of the encapsidation of a finite number of dsRNA segments in a capsid of 9.8 × 106 daltons. Although one to three M dsRNA segments were encapsidated in a single virion, no particles were detected with more than one H dsRNA segment per virion.