A FUNCTIONALLY CONSERVED HOMOLOGY OF THE DROSOPHILA SEGMENT POLARITY GENE-HH IS EXPRESSED IN TISSUES WITH POLARIZING ACTIVITY IN ZEBRAFISH EMBRYOS
A FUNCTIONALLY CONSERVED HOMOLOGY OF THE DROSOPHILA SEGMENT POLARITY GENE-HH IS EXPRESSED IN TISSUES WITH POLARIZING ACTIVITY IN ZEBRAFISH EMBRYOS
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DOI：
10.1016/0092-8674(93)90628-4
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发表时间：
1993-12-31
期刊：
影响因子：
64.5
通讯作者：
INGHAM, PW
中科院分区：
文献类型：
--
作者：
KRAUSS, S;CONCORDET, JP;INGHAM, PW
片段极性基因hedgehog(HH)编码一种新的信号蛋白，在发育中的果蝇胚胎中介导局部细胞-细胞相互作用。在这里，我们描述了斑马鱼Brachydanio rerio中HH相关基因家族的存在。其中，sonic hedgehog(Shh)基因在脊索、底板和后鳍中胚层表达，这些组织与各种脊椎动物胚胎的极化活动有关。Shh在斑马鱼突变体中影响轴结构的表达模式，以及其在正常胚胎中异位表达的后果，与Shh在底板诱导中的作用是一致的。通过在转基因果蝇胚胎中表达shh，我们还证明了鱼类和果蝇hh基因之间具有很强的功能保守性。
The segment polarity gene hedgehog (hh) encodes a novel signaling protein that mediates local cell-cell interactions in the developing Drosophila embryo. Here we describe the existence of an hh-related gene family in the zebrafish, Brachydanio rerio. One of these genes, sonic hedgehog (shh), is expressed in the notochord, floor plate, and posterior fin mesoderm, tissues associated with polarizing activities in various vertebrate embryos. The pattern of shh expression in zebra-fish mutants affecting axial structures, together with the consequences of its ectopic expression in normal embryos, is consistent with a role for shh in floor plate induction. By expressing shh in transgenic Drosophila embryos, we also demonstrate a strong functional conservation between the fish and fly hh genes.