Fluvastatin accelerates re-endothelialization impaired by local sirolimus treatment
Fluvastatin accelerates re-endothelialization impaired by local sirolimus treatment
复制标题
DOI：
10.1016/j.ejphar.2009.04.006
复制
发表时间：
2009-06-10
影响因子：
5
通讯作者：
Sata, Masataka
中科院分区：
文献类型：
--
作者：
Fukuda, Daiju;Enomoto, Soichiro;Sata, Masataka
西罗莫司洗脱支架减少经皮冠状动脉介入治疗后再狭窄。然而.越来越多的证据表明，西罗莫司可能影响再内皮化，导致晚期血栓形成。他汀类药物对内皮细胞有保护作用。最近，他汀类药物被报道增加循环内皮祖细胞（EPCs）的数量，并加速血管损伤后的再内皮化。在这里，我们测试了氟伐他汀对局部西罗莫司治疗后的油再内皮化具有有益作用的假设。我们对野生型小鼠和骨髓嵌合小鼠的股动脉两侧进行了金属丝介导的血管损伤。西罗莫司（100 μ g）或DMSO局部给药于损伤动脉的血管周围区域。所有小鼠在手术前一周开始通过管饲法接受氟伐他汀（5 mg/kg/天）或溶媒，直至处死。术后4周，通过CD 31阳性面积百分比测定，西罗莫司治疗组小鼠动脉的再内皮化显著低于DMSO治疗组（P
Sirolimus-eluting stent reduces restenosis after percutaneous coronary intervention. However. accumulating evidence suggests that sirolimus potentially affects re-endothelialization, leading to late thrombosis. Statins have protective effects on endothelium. Recently, statins are reported to increase the number of circulating endothelial progenitor cells (EPCs) and accelerate re-endothelialization after vascular injury. Here, we tested the hypothesis that fluvastatin has beneficial effect oil re-endothelialization after local sirolimus treatment. We performed wire-mediated vascular injury to both sides of femoral arteries of wild-type mice and bone marrow chimeric mice. Either sirolimus (100 mu g) or DMSO was administered locally to the perivascular area of the injured arteries. All mice received either fluvastatin (5 mg/kg/day) or vehicle by gavage starting at one week before the surgery until sacrifice. At 4 weeks after the surgery, re-endothelialization of the sirolimus-treated artery was significantly less than that of DMSO-treated one in the vehicle-treated mice as determined by the percentage of CD31-positive area (P