Monoclonal antibodies specific to chicken iodopsin.
Monoclonal antibodies specific to chicken iodopsin.
复制标题
鸡碘紫红质特异性单克隆抗体。
DOI：
10.1016/0042-6989(89)90184-3
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发表时间：
1989
期刊：
影响因子：
1.8
通讯作者：
Ebrey,TG
中科院分区：
文献类型：
--
作者：
Chen,JG;Nakamura,T;Matsumoto,H;Ebrey,TG
提出了从Hyrilobulin细胞的单克隆抗体（mAB）和筛选纯化的锥色素，碘视蛋白，从鸡视网膜。用四种不同的方法检测这些抗体：（1）斑点免疫结合试验;（2）酶联免疫吸附试验（ELISA）;（3）一维免疫印迹（4）二维免疫印迹。鉴定了三类抗体产生细胞系。一类产生对碘视蛋白特异性的mAB。来自第二类的单克隆抗体与碘视蛋白和可能的其他三种锥状色素之一发生交叉反应。来自第三类的单抗可能与鸡视网膜中的所有四种锥状色素发生交叉反应。选择来自所有这些类别的杂交瘤细胞系的mAb，使得它们不与视紫红质交叉反应。二维免疫印迹表明，碘视蛋白具有比视紫红质高得多的等电点，如从人视杆和视锥色素的已知氨基酸序列所建议的。
Monoclonal antibodies (mABs) from hyridoma cells were raised and screened with a purified cone pigment, iodopsin, from the chicken retina. Four different methods were used to test these antibodies:(1) dot-immunobinding assay;(2) enzyme-linked immunoabsorbent assay (ELISA);(3) one dimensional immunoblotting(4) two dimensional immunoblotting. Three classes of antibody producing cell lines were identified. One class produces a mAB specific to iodopsin. The mAB from the second class crossreacts with iodopsin and probably one of the other three cone pigments. The mAB from the third class probably crossreacts with all the four cone pigments in the chicken retina. The mABs from all these classes of hybridoma cell lines were selected so that they do not crossreact with rhodopsin. Two dimensional immunoblotting indicated that iodopsin has a much higher isoelectric point than rhodopsin, as suggested from the known amino acid sequences of human rod and cone pigments.