STUDIES ON THE DEVELOPMENT OF ANTI-SCHISTOSOMULAR SURFACE ANTIBODIES BY MICE EXPOSED TO IRRADIATED CERCARIAE, ADULTS AND/OR EGGS OF S-MANSONI
STUDIES ON THE DEVELOPMENT OF ANTI-SCHISTOSOMULAR SURFACE ANTIBODIES BY MICE EXPOSED TO IRRADIATED CERCARIAE, ADULTS AND/OR EGGS OF S-MANSONI
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DOI：
10.1111/j.1365-3024.1983.tb00764.x
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
ANDREWS, BJ
中科院分区：
文献类型：
--
作者：
BICKLE, QD;FORD, MJ;ANDREWS, BJ
抗体结合到童虫表面和介导体外嗜酸性粒细胞依赖的细胞毒性的童虫的水平进行了研究，并在体内水平的尾蚴的挑战，在感染照射尾蚴，未照射的单或混合性别的Carcariae，或注射鸡蛋的阻力进行了比较。通过计数粘附于机械转化的小鼠胚泡的携带IgG-Fc受体的细胞（P388 D1细胞）的数量来评估抗体结合。暴露于辐照尾蚴后1-2周采集的血清出现显著的粘附水平，此后粘附水平逐渐增加，并因重复暴露而增强。两性感染的血清在第5-8周之间显示出结合活性的急剧增加，并且对于单一性别感染，在第10周之前有稳定的上升，然后在第10-12周之间急剧上升。每周注射一次鸡蛋，血清结合活性稳步上升。还测试了在攻击前采集的血清在体外介导异源大鼠腹膜渗出细胞的嗜酸性粒细胞富集制剂杀死童虫的能力。所有血清都有显著的杀灭作用，但最大的致死活性出现在注射卵、慢性感染和200例男性尾蚴感染组的血清中。这种排名没有相关的体内抵抗挑战，评估蠕虫恢复，鸡蛋注射和单一性别感染组未能表现出显着的阻力。
Levels of antibody binding to the schistosomulum surface and mediating in vitro eosinophil-dependent cytotoxicity of schistosomula were studied and compared to the in vivo levels of resistance of cercarial challenge in mice infected with irradiated cercariae, unirradiated carcariae of single or mixed sex, or injected with eggs. Antibody-binding was assessed by counting the number of IgG-Fc-receptor bearing cells (P388D1 cells) adhering to mechanically-transformed schistosomula. Significant levels of adherence occurred with sera taken 1-2 wk following exposure to irradiated cercariae, the level increasing gradually thereafter and being enhanced by repeated exposure. Sera from the bisexual infection showed a dramatic increase in binding activity between weeks 5-8, and with the single sex infections there was a steady rise up to wk 10 followed by a sharp rise between wk 10-12. Weekly injections of eggs produced a steady rise in serum binding activity. Sera taken just before challenge were also tested for their ability to mediate killing of schistosomula by eosinophil-enriched preparations of heterologous rat peritoneal exudate cells in vitro. Significant levels of killing occurred with all sera, but the greatest lethal activity was found in sera from the egg-injected, chronically-infected and 200 male cercariae-infected groups. This ranking did not correlate with in vivo resistance against challenge as assessed by worm recovery, the egg-injected and single sex-infected groups failing to manifest significant resistance.