Graphene Quantum Dots for Radiotherapy
Graphene Quantum Dots for Radiotherapy
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用于放射治疗的石墨烯量子点
DOI：
10.1021/acsami.7b18975
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发表时间：
2018-05-02
影响因子：
9.5
通讯作者：
Ge, Shengfang
中科院分区：
文献类型：
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作者：
Ruan, Jing;Wang, Ying;Ge, Shengfang
放射治疗是一种广泛应用于临床的肿瘤治疗方法，但其治疗效果在很大程度上受到各种因素的影响。目前，人们正在努力寻找有效和安全的放射增敏剂。纳米放射增敏剂由于其高的肿瘤组织摄取和二次电子产生率而成为提高肿瘤放射治疗效果的理想选择。本文通过氧化石墨烯的光芬顿反应制备并纯化了具有良好氧化应激响应和显著高的光毒性的高度氧化的石墨烯量子点（GQD）。通过对大肠癌细胞周期和凋亡程度的监测，系统评价GQD辐射增敏效应，并初步探讨GQD辐射增强细胞凋亡的可能机制。我们的数据表明，GQD与电离辐射（IR）的协同作用可以显著增强细胞的G2/M期阻滞，抑制细胞增殖，并促进凋亡。这主要是由于GQDs与IR结合产生过量的活性氧，从而激活肿瘤相关调节蛋白，导致肿瘤细胞凋亡。本研究提示GQDs可作为一种新型的纳米放射增敏剂应用于肿瘤放射治疗。
Radiation therapy is a kind of tumor treatment that has been widely employed in clinics, but its therapeutic effect is largely hampered by various factors. Currently, considerable efforts are being made in the search for effective and safe radiosensitizers. A nano-radiosensitizer is an ideal choice for improving the effects of tumor radiotherapy due to its high degree of tumor tissue uptake and secondary electrons' productivity. Herein, highly oxidized graphene quantum dots (GQDs) with a good oxidative stress response and significantly high phototoxicity were prepared and purified via the photo-Fenton reaction of graphene oxide. The enhanced radiosensitization effects were systematically evaluated by monitoring colorectal carcinoma cell cycle and the degree of apoptosis, and the possible mechanism of the GQD irradiating enhancement of cell apoptosis was preliminarily investigated. Our data showed that the GQD synergy with ionizing radiation (IR) could noticeably enhance the G2/M stage arrest of cells, inhibit cell proliferation, and improve apoptosis. This is mainly due to the overproduction of reactive oxygen species by GQDs in combination with the IR, which activates the apoptosis-related regulation proteins and results in tumor cell apoptosis. This study suggests that the GQDs can act as a new nano-radiosensitizer in tumor radiotherapy.