Photomotile responses of Blepharisma japonicum I: Action spectra determination and time-resolved fluorescence of photoreceptor pigments
Photomotile responses of Blepharisma japonicum I: Action spectra determination and time-resolved fluorescence of photoreceptor pigments
复制标题
Blepharisma japonicum I 的光动力响应：光感受器色素的作用光谱测定和时间分辨荧光
DOI：
10.1016/1011-1344(87)80007-6
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发表时间：
1987
影响因子：
5.4
通讯作者：
V. Passarelli
中科院分区：
文献类型：
--
作者：
Patrizia Scevoli;F. Bisi;G. Colombetti;F. Ghetti;F. Lenci;V. Passarelli
采用显微摄像技术研究了日本睑螨的光诱导回避反应。光反应细胞的百分比和刺激开始和反应之间的滞后时间被测量为注量率和光刺激波长的函数。作用光谱表明，红色素眼睑色素是这种光响应的感光色素。用相位调制荧光分光光度计测定了乙醇提取色素和冷挤压色素颗粒的荧光寿命和量子产率。颗粒中色素的荧光寿命和量子产率是乙醇溶液中色素的一半。这可能是在协议的假设，即眼睑色素是所观察到的光行为的感光色素。
The light-induced avoiding reaction ofBlepharisma Japonicumwas investigated by means of microscope videorecording techniques. The percentage of photoreacting cells and the lag time between stimulus onset and reaction were measured as a function of the fluence rate and of the light stimulus wavelength. Action spectra indicate that the red pigment blepharismin is the photoreceptor pigment for this photoresponse. The fluorescence lifetimes and quantum yields of ethanol-extracted pigments and cold-extruded pigment granules were measured by means of a phase-modulation spectrofluorometer. The fluorescence lifetime and quantum yield of the pigment in granules are half those of pigments in ethanol solution. This could be in agreement with the hypothesis that blepharismin is the photoreceptor pigment for the observed photobehaviour.
原生动物和相关光感受器：分子方面。
DOI：
10.1146/annurev.bb.12.060183.000343
发表时间：
1983
期刊：
Annual review of biophysics and bioengineering
影响因子：
--
作者：
Song,PS
通讯作者：
Song,PS