OPTICAL ROTATORY DISPERSION OF 2 BETA STRUCTURES
OPTICAL ROTATORY DISPERSION OF 2 BETA STRUCTURES
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DOI：
10.1016/s0006-291x(67)80063-9
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发表时间：
1967-01-01
影响因子：
3.1
通讯作者：
POTTER, J
中科院分区：
文献类型：
--
作者：
FASMAN, GD;POTTER, J
随着酶、抗体等的拓扑结构通过x射线衍射研究、活性位点研究和抗原-抗体相互作用变得明显，蛋白质构象评价的重要性也随之增加。光学旋转色散（ORD）在估计蛋白质和合成多肽的构象方面发挥了重要作用（Blout, 1960； Urnes和Doty, 1961; Fasman, 1963）。最近在溶液中发现了~结构的OR/) (Davidson et al., 1966; Sarkar and Doty, 1966)，现在已经可以使用三种基本结构的ORD模式，a，~和随机线圈，被认为存在于蛋白质中。蛋白质有三种构象（Pauling和Corey, 1951; Rudall, 1952），分子间平行和反平行氢键片，分子内氢键交叉结构。它们的区分主要是基于红外二色性研究（Miyazawa and Blout, 1961; Krimm, 1962），但还不能通过红外光谱进行区分
The importance of the conformational evaluation of proteins increases as the topology of enzymes, antibodies, etc. becomes evident through X-ray diffraction studies, active site investigations and antigen-antibody interactions. Optical rotatory dispersion (ORD) has played a significant role in the estimation of the conformation of proteins and synthetic polypeptides (Blout, 1960; Urnes and Doty, 1961; Fasman, 1963). The recent discovery of the OR/) of the~ structure in solution (Davidson et al., 1966; Sarkar and Doty, 1966) has now made available the ORD patterns of the three basic structures, a,~ and random coil, thought to exist in proteins. Three~ conformations have been postulated for proteins (Pauling and Corey, 1951; Rudall, 1952), the intermolecular parallel and antiparallel hydrogen-bonded sheets, and the intramolecular hydrogen bonded cross~ structures. Their differentiation has been based mainly on infrared dichroism studies (Miyazawa and Blout, 1961; Krimm, 1962) and has not yet been possible by ORD. We wish to report the ORD spectra of films of 1