Processing of sting pheromone and its components in the antennal lobe of the worker honeybee.
Processing of sting pheromone and its components in the antennal lobe of the worker honeybee.
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DOI：
10.1016/j.jinsphys.2008.03.004
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发表时间：
2008-05
影响因子：
2.2
通讯作者：
Shunpeng Wang;Katsushige Sato;M. Giurfa;Shaowu Zhang
中科院分区：
文献类型：
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作者：
Shunpeng Wang;Katsushige Sato;M. Giurfa;Shaowu Zhang
在西方蜜蜂中，由刺腺产生的刺信息素在协调防御行为中起着重要作用。这种信息素是由几种成分组成的混合物。人们对蜜蜂大脑中蜇刺信息素加工的神经基质知之甚少。在这里，我们调查了八个组成部分（五个醋酸酯和三个醇）的刺信息素，并由真实的蜜蜂蜇伤的触角叶（AL）的工人蜜蜂的水平引起的神经活动。我们使用在体内钙成像记录气味诱导的神经活动的22个确定肾小球在AL。我们发现，乙酸酯主要激活内侧肾小球，而醇主要激活外侧背侧肾小球。螫制剂引起的肾小球模式，这是明显有别于那些个别信息素成分。没有特定区域的成像AL被发现处理蜇信息素或其任何组成部分。在一个假定的蜜蜂嗅觉空间的进一步分析表明，刺准备引起的神经活动不能线性预测的信息素成分，这些组件没有明确地从非刺信息素气味分开。我们的结论是，刺痛信息素处理的工人蜜蜂AL遵循相同的原则，一般的气味，使气味的化学结构是肾小球激活的主要决定因素，而不是他们的信息素值。然而，我们不能排除，刺信息素表征相对于其组分的独特性构成了这种物质的专门神经处理策略的一种形式。
In the honeybee Apis mellifera, a sting pheromone produced by sting glands plays an important role in coordinating defensive behavior. This pheromone is a blend constituted by several components. Little is known about the neural substrates underlying sting pheromone processing in the bee brain. Here, we investigated the neural activity elicited by eight components (five acetates and three alcohols) of the sting pheromone, and by real bee stings at the level of the antennal lobe (AL) of worker honeybees. We used in vivo calcium imaging to record odor-induced neural activity of 22 identified glomeruli in the AL. We found that acetates mainly activated medial glomeruli while alcohols mainly activated lateral dorsal glomeruli. The sting preparation evoked a glomerular pattern that was clearly distinct from those of individual pheromone components. No particular region of the imaged AL was found to process sting pheromone or any of its components. Further analyses in a putative honeybee olfactory space showed that the neural activity elicited by sting preparation cannot be linearly predicted by those of pheromone components and that such components are not clearly separated from non-sting pheromone odors. We conclude that sting pheromone is processed in the worker honeybee AL following the same principles of general odors so that the chemical structure of odorants is the main determinant of glomerular activation, rather than their pheromonal values. We cannot exclude, however, that the distinctness of sting-pheromone representation with respect to that of its components constitutes a form of specialized neural processing strategy for this kind of substance.