Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.
Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.
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DOI：
10.1016/j.mimet.2015.12.004
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发表时间：
2016-01
影响因子：
2.2
通讯作者：
Mostowy S
中科院分区：
文献类型：
--
作者：
Larrouy-Maumus G;Clements A;Filloux A;McCarthy RR;Mostowy S
革兰氏阴性菌脂质A的纯化和表征是繁琐和耗时的。在这里，我们报告了一种快速，灵敏的方法来确定脂质A直接对完整的细菌，没有任何化学处理或纯化，使用非典型溶剂系统，以溶解基质结合MALDI-TOF质谱。革兰氏阴性菌脂质A可以通过MALDI-TOF MS在完整细菌上直接检测。不需要提取或纯化步骤。该方法快速（< 5 min），仅需要约104-105个细菌。
The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry. Gram-negative bacterial lipid A can be directly detected on intact bacteria by MALDI-TOF MS. No extractions or purification steps are required. The method is rapid (< 5 min), and only requires about 104–105 bacteria.