Evidence that stem cells reside in the adult Drosophila midgut epithelium
Evidence that stem cells reside in the adult Drosophila midgut epithelium
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DOI：
10.1038/nature04371
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发表时间：
2006-01-26
期刊：
影响因子：
64.8
通讯作者：
Perrimon, N
中科院分区：
文献类型：
--
作者：
Micchelli, CA;Perrimon, N
成体干细胞在整个生命过程中维持器官系统，并促进损伤或疾病后的修复（1）。干细胞和祖细胞分裂的一个基本特性是保持增殖状态或产生分化的子细胞的能力（2）;然而，目前对调节这些过程之间平衡的信号知之甚少。在这里，我们的特点是在成年果蝇中肠增殖细胞室。使用遗传镶嵌分析，我们表明，分化的上皮细胞来自一个共同的血统。此外，我们表明，减少Notch信号导致中肠祖细胞的数量增加，而激活的Notch途径导致增殖减少。因此，中肠祖细胞的默认状态是增殖，其通过Notch信号传导途径被抑制。鉴定、操纵和遗传追踪中肠细胞谱系的能力将导致发现调节胃肠道中干细胞和祖细胞生物学的其他基因。
Adult stem cells maintain organ systems throughout the course of life and facilitate repair after injury or disease(1). A fundamental property of stem and progenitor cell division is the capacity to retain a proliferative state or generate differentiated daughter cells(2); however, little is currently known about signals that regulate the balance between these processes. Here, we characterize a proliferating cellular compartment in the adult Drosophila midgut. Using genetic mosaic analysis we demonstrate that differentiated cells in the epithelium arise from a common lineage. Furthermore, we show that reduction of Notch signalling leads to an increase in the number of midgut progenitor cells, whereas activation of the Notch pathway leads to a decrease in proliferation. Thus, the midgut progenitor's default state is proliferation, which is inhibited through the Notch signalling pathway. The ability to identify, manipulate and genetically trace cell lineages in the midgut should lead to the discovery of additional genes that regulate stem and progenitor cell biology in the gastrointestinal tract.