Cellular and Molecular Responses Unique to Major Injury Are Dispensable for Planarian Regeneration
Cellular and Molecular Responses Unique to Major Injury Are Dispensable for Planarian Regeneration
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DOI：
10.1016/j.celrep.2018.11.004
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发表时间：
2018-11-27
期刊：
影响因子：
8.8
通讯作者：
Reddien, Peter W.
中科院分区：
文献类型：
--
作者：
Tewari, Aneesha G.;Stern, Sarah R.;Reddien, Peter W.
人们对再生的基本要求知之甚少。涡虫在损伤后可以强健地再生所有组织，包括干细胞、位置信息和一组统称为“缺失组织”或“再生”反应的细胞和分子反应。卵泡抑素是一种细胞外激活素抑制剂的编码物，它对于头部截肢后缺失的组织反应和随后的再生是必需的。我们发现，无论伤口情况如何，卵泡抑素都是缺失组织反应所必需的，但只有在头部截肢后才会导致再生失败。这种头部再生失败涉及卵泡抑素介导的Wnt信号在伤口的调节，而不是减少缺失组织反应的结果。所有被测试的再生情况，包括头部再生，都可能发生有缺陷的缺失组织反应，但速度较慢。我们的研究结果表明，由大损伤引起的主要细胞和分子程序具有加速再生的功能，但对于再生本身是必不可少的。
The fundamental requirements for regeneration are poorly understood. Planarians can robustly regenerate all tissues after injury, involving stem cells, positional information, and a set of cellular and molecular responses collectively called the "missing tissue'' or "regenerative'' response. follistatin, which encodes an extracellular Activin inhibitor, is required for the missing tissue response after head amputation and for subsequent regeneration. We found that follistatin is required for the missing tissue response regardless of the wound context, but causes regeneration failure only after head amputation. This head regeneration failure involves follistatin-mediated regulation of Wnt signaling at wounds and is not a consequence of a diminished missing tissue response. All tested contexts of regeneration, including head regeneration, could occur with a defective missing tissue response, but at a slower pace. Our findings suggest that major cellular and molecular programs induced specifically by large injuries function to accelerate regeneration but are dispensable for regeneration itself.