Solute Transport in Engineered Living Materials Using Bone‐Inspired Microscale Channel Networks
Solute Transport in Engineered Living Materials Using Bone‐Inspired Microscale Channel Networks
复制标题
使用骨启发的微尺度通道网络进行工程活性材料中的溶质运输
DOI：
10.1002/adem.202301032
复制
发表时间：
2023
影响因子：
3.6
通讯作者：
Hernandez, Christopher J.
中科院分区：
文献类型：
--
作者：
van Wijngaarden, Ellen W.;Bratcher, Samantha;Lewis, Karl J.;Hernandez, Christopher J.
工程生物材料（ELMs）是一类新兴的材料，由活细胞合成和/或填充，以实现新的功能，包括自我修复和传感。在长时间内为ELM内的活细胞提供营养仍然是限制ELM使用寿命的主要技术挑战。骨骼通过由微米级孔隙组成的纳米级通道网络输送营养物质，从而维持活细胞数十年。骨中的营养转移通过材料在常规使用期间经历的机械负荷实现。在此，可以在ELM中使用的通道和孔隙网络的几何特征以骨骼中观察到的方式进行鉴定，以允许机械加载，从而能够将营养物质输送到驻留细胞群。当微尺度孔隙网络中的变形超过连接通道的体积时，发生传输。计算模型表明，在更大的加载幅度和更低的加载频率，运输增强。使用微流控系统的实验证实了计算结果。在研究结果中，为能够持续向材料内的活细胞输送营养物质的通道-孔隙网络提供了定量设计原则。
Engineered living materials (ELMs) are an emerging class of materials that are synthesized and/or populated by living cells to achieve novel functionalities including self‐healing and sensing. Providing nutrients to living cells within an ELM over prolonged periods remains a major technical challenge that limits the service life of ELMs. Bone maintains living cells for decades by delivering nutrients through a network of nanoscale channels punctuated by microscale pores. Nutrient transfer in bone is enabled by mechanical loading experienced by the material during regular use. Herein, the geometric traits of the network of channels and pores that can be used in ELMs to allow mechanical loading to enable nutrient delivery to resident cell populations are identified in a manner seen in bone. Transport occurs when deformation in the microscale pore network exceeds the volume of the connecting channels. Computational models show that transport is enhanced at greater loading magnitudes and lower loading frequencies. The computational results are confirmed using experiments with microfluidic systems. In the findings, quantitative design principles are provided for channel‐pore networks capable of sustained delivery of nutrients to living cells within materials.