Protective effect of feruloylated oligosaccharides on dextran sulfate sodium-induced ulcerative colitis in rats
Protective effect of feruloylated oligosaccharides on dextran sulfate sodium-induced ulcerative colitis in rats
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DOI：
10.1002/fft2.140
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发表时间：
2022-09-01
期刊：
影响因子：
9.9
通讯作者：
Wang, Jing
中科院分区：
文献类型：
--
作者：
Gong, Lingxiao;Hu, Linlin;Wang, Jing
阿魏酸寡糖（ferulylated oligosaccharides，FOs）是由阿魏酸和阿拉伯聚糖组成，已被证实具有与溃疡性结肠炎（ulcerative colitis，UC）发病机制密切相关的有益特性。本研究旨在探讨FOs对慢性UC大鼠的保护作用及其调控机制。研究表明，FOs通过抗氧化、抑制炎症反应、改善肠道屏障和调节肠道微生物群整体结构等药理学机制的组合来减轻慢性UC。FO通过下调结肠组织中促炎细胞因子IL-1 β、IL-6和TNF-α的相对表达而显示免疫调节作用。此外，FOs还具有抗氧化活性，可提高SOD活性，降低MDA含量、iNOS活性和NO含量。在肠屏障方面，FOs通过增强紧密连接蛋白claudin-3、occluding和ZO-1的表达，恢复肠屏障的超微结构，抑制肠屏障损伤。此外，FOs还可以部分恢复DSS诱导的UC大鼠肠道生物多样性，增加阿克曼氏菌和罗斯拜瑞氏菌等有益菌的物种丰富度。这些发现为FOs介导的慢性UC缓解提供了新的见解，并将促进慢性UC的预防策略。
Feruloylated oligosaccharides (FOs), comprising ferulic acid and arabinoxylans, have already been proved to possess beneficial properties that are closely related to the pathogenesis of ulcerative colitis (UC). This study aims at investigating the protective effect of FOs on chronic UC rats and its regulatory mechanisms. The study demonstrated that FOs alleviated the chronic UC through a combination of pharmacological mechanisms, including antioxidant, inhibiting the inflammatory response, improving the gut barrier and modulating the overall structure of gut microbiota. FOs showed immunomodulatory effect by downregulating the relative expression of proinflammatory cytokines IL-1 beta, IL-6, and TNF-alpha in colon tissue. In addition, the FOs exhibited antioxidant activity by increasing SOD activity but reducing MDA content, iNOS activity and NO content. In terms of intestinal barrier, FOs inhibited gut barrier damages by enhancing tight junction protein expression (claudin-3, occluding, and ZO-1) and recovering the ultrastructure of intestinal barrier. Moreover, FOs also could partially restore the intestinal biodiversity in the DSS-induced UC rats and increased the species richness of beneficial bacteria such as Akkermansia and Roseburia. These findings provide novel insights into the FOs-mediated alleviation of chronic UC and will facilitate the preventive strategies for chronic UC.