Changes in growth performance, haematological parameters, hepatopancreas histopathology and antioxidant status of pacific white shrimp (Litopenaeus vannamei) fed oxidized fish oil: Regulation by dietary myo-inositol
Changes in growth performance, haematological parameters, hepatopancreas histopathology and antioxidant status of pacific white shrimp (Litopenaeus vannamei) fed oxidized fish oil: Regulation by dietary myo-inositol
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DOI：
10.1016/j.fsi.2019.02.023
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发表时间：
2019-05-01
影响因子：
4.7
通讯作者：
Liu, Yongjian
中科院分区：
文献类型：
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作者：
Chen, Shijun;Zhuang, Zhenxiao;Liu, Yongjian
通过58d饲养试验，研究了饲料中添加肌醇(MI)对凡纳滨对虾生长性能、血液学指标、肝胰腺组织病理学和抗氧化状态的影响。对照日粮中添加新鲜鱼油(FFO)，不添加MI。其他4种日粮均含有两种氧化水平的OfO(过氧化值：133.2和268.7 meq kg(-1))，分别添加或不添加MI0+L、MI0+H、MI200+L和MI200+H。结果表明，与对照组相比，添加Ofo组(不添加MI)具有更好的生长性能和更低的全身肌醇含量。添加MI显著提高了高氧化鱼油(H0F0)组的全身肌醇含量，也降低了低氧化鱼油(LOFO)组的全身脂肪含量。此外，补充Ofo和MI显著影响肌肉的脂肪酸组成。HOFO引起对虾肝胰腺的严重组织病理学变化，添加MI后这种变化略有减轻。补充MI还能提高血清总蛋白(TP)含量和碱性磷酸酶(AKP)活性，降低血清天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)活性。摄入Ofo增加了血清或肝胰腺中的脂质过氧化和蛋白质氧化水平，补充MI可部分改善这一水平。抗氧化酶活性因Ofo和MI的不同表现出不同的表达模式。此外，HOFO还能显著提高抗氧化性基因铁蛋白(FT)、硫氧还蛋白(Trx)、GPx、谷胱甘肽S转移酶(Gst)和过氧化氢酶(CAT)的基因表达水平，降低过氧化还蛋白(Prx)的表达，其中GPx和Prx的表达因MI的补充而增加。由此可见，饲料中添加Ofo刺激了凡纳滨对虾的生长性能，但也诱导了氧化应激，对肝胰腺造成了损伤。补充膳食MI可部分缓解Ofo带来的负面影响。
A 58-day feeding trial was conducted to evaluate the effects of dietary myo-inositol (MI) supplementation on growth performance, haematological parameters, hepatopancreas histopathology and antioxidant status of Litopenaeus vannamei fed with oxidized fish oil (OFO). Control diet contained fresh fish oil (FFO) without MI supplementation. The other four diets contained two oxidation levels of OFO (peroxide value: 133.2 and 268.7 meq kg(-1)) with or without 200 mg MI kg(-1) diets (MI0+L, MI0+ H, MI200 + L and MI200 + H). Results showed that OFO-supplemented groups (without MI supplementation) showed better growth performance and lower whole-body inositol content when opposed to control group. MI supplementation significantly improved whole-body inositol content in high-oxidized fish oil (H0F0) groups, and also reduced whole-body lipid in low oxidized fish oil (LOFO) groups. Moreover, Supplementation of OFO and MI markedly hit the fatty acid profile of muscle. HOFO caused severe histopathological changes in hepatopancreas of shrimp, which slightly alleviated by MI supplementation. MI supplementation also grew the total protein (TP) content and alkaline phosphatase (AKP) activity and decreased the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of serum in OFO-supplemented groups. Ingestion of OFO increased levels of lipid peroxidation and protein oxidation in serum or hepatopancreas, which partly ameliorated by MI supplementation. Activities of antioxidant enzymes exhibited different expression patterns because of OFO and MI. In addition, HOFO markedly increased mRNA expression levels of antioxidant genes including ferritin (FT), thioredoxin (Trx), GPX, glutathione S-transferase (GST) and catalase (CAT) and decreased peroxiredoxin (Prx) expression, in which expression of GPX and Prx were increased owing to MI supplementation. Therefore, it suggested that dietary OFO stimulated growth performance, but also induced oxidative stress and caused impairment to hepatopancreas in L. vannamei. The negative impact brought about by OFO was partially mitigated by dietary MI supplementation.