Removing symbiotic Wolbachia bacteria specifically inhibits oogenesis in a parasitic wasp
Removing symbiotic Wolbachia bacteria specifically inhibits oogenesis in a parasitic wasp
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DOI：
10.1073/pnas.101304298
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发表时间：
2001-05-22
影响因子：
11.1
通讯作者：
Boulétreau, M
中科院分区：
文献类型：
--
作者：
Dedeine, F;Vavre, F;Boulétreau, M
沃尔巴克氏菌是生活在各种无脊椎动物细胞中的细菌，它们会对生理和生殖产生广泛的影响。本文研究了沃尔巴克氏菌感染对寄生黄蜂的影响。在测试的13个种群中，所有个体都被证明感染了沃尔巴克氏菌。用抗生素去除沃尔巴克氏菌产生了一个完全意想不到的效果--非共生的雌性黄蜂完全不能产生成熟的卵母细胞，因此不能繁殖。相比之下，处理过的柑橘青霉的卵子发生没有受到影响，这是一种关系密切的物种，没有沃尔巴克氏菌。自然共生个体和治愈个体在雄性育性、活动能力和大小等成虫性状上没有差异，表明其对卵子发生的影响具有高度的特异性。我们认为，我们研究中使用的治疗方法的间接影响(抗生素毒性或有毒物质的产生)不太可能解释女性不育的原因，我们提出的结果表明，女性的卵母细胞产量和沃尔巴克氏菌密度之间存在直接关系。我们得出结论，沃尔巴克氏菌对于这些烟粉虱品系的卵子发生是必要的，这种关联似乎是节肢动物-沃尔巴克氏菌群丛中从兼性共生向强制性共生转变的第一个例子。
Wolbachia are bacteria that live in the cells of various invertebrate species to which they cause a wide range of effects on physiology and reproduction. We investigated the effect of Wolbachia infection in the parasitic wasp, Asobara tabida Nees (Hymenoptera, Braconidae). In the 13 populations tested, all individuals proved to be infected by Wolbachia. The removal of Wolbachia by antibiotic treatment had a totally unexpected effect-aposymbiotic female wasps were completely incapable of producing mature oocytes and therefore could not reproduce. In contrast, oogenesis was not affected in treated Asobara citri, a closely related species that does not harbor Wolbachia. No difference between natural symbiotic and cured individuals was found for other adult traits including male fertility, locomotor activity, and size, indicating that the effect on oogenesis is highly specific. We argue that indirect effects of the treatments used in our study (antibiotic toxicity or production of toxic agents) are very unlikely to explain the sterility of females, and we present results showing a direct relationship between oocyte production and Wolbachia density in females. We conclude that Wolbachia is necessary for oogenesis in these A. tabida strains, and this association would seem to be the first example of a transition from facultative to obligatory symbiosis in arthropod-Wolbachia associations.