The alterations of gut microbiota in mice with chronic pancreatitis
The alterations of gut microbiota in mice with chronic pancreatitis
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慢性胰腺炎小鼠肠道菌群的变化
DOI：
10.21037/atm.2019.08.18
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发表时间：
2019-09-01
影响因子：
--
通讯作者：
Li, Ling
中科院分区：
文献类型：
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作者：
Han, Man-Man;Zhu, Xiang-Yun;Li, Ling
背景资料：肠道微生物组的变化与炎症，代谢和恶性疾病有关，目前还没有研究评估慢性胰腺炎（CP）小鼠的肠道微生物组。方法：雄性C57 BL/6小鼠16只，随机分为两组，分别腹腔注射生理盐水（正常对照组，CT组）和乙醇+雨蛙肽（实验组，CP组）6周。整个过程中测量的体重。组织学检查CP指数以验证CP诱导。将从结肠样品提取的DNA用于16 S rRNA基因的细菌V4区的Illumina HiSeq测序，并使用微生物生态学定量分析（QIIME）进行分析。结果：CP组小鼠肠道菌群的丰富度和多样性显著降低，毛螺菌科（Lachnospiraceae_NK4A136）、瘤胃梭菌属（Ruminiclostridium）和罗斯拜瑞氏菌属（Roseburia）的相对丰度下降，拟杆菌属（Bacteroides）和阿罗普雷沃菌属（Alloprevotella）的相对丰度增加。微生物群落水平的表型分析显示CP组和CT组之间在9种表型（需氧、厌氧、含移动的元素、兼性厌氧、生物膜形成、革兰氏阴性、革兰氏阳性、潜在致病性和应激耐受性）上存在显著差异。
Background: The changes of intestinal microbiome are associated with inflammatory, metabolic, and malignant disorders, and there are no studies assessing the intestinal microbiota of mice with chronic pancreatitis (CP). Thus, we aim to investigate the variations in diversity, composition and function of intestinal microbiota in CP mice.Methods: Sixteen male C57BL/6 mice were randomly selected, and divided into two groups, treated intraperitoneally with saline (normal control group, CT group) or ethanol + cerulein (experimental group, CP group) for 6 weeks. Body weight as measured in entire processes. Histopathological examination of CP index was conducted to verify the CP induction. Extracted DNA from colon samples was used for Illumina HiSeq sequencing of the bacterial V4 region of 16S rRNA gene and analyzed using Quantitative Insights Into Microbial Ecology (QIIME). Functional profiling of microbial communities was predicted with BugBase.Results: Significant alterations of the gut microbiota were found in the CP mice compared to CT groups, as revealed by significant decrease in bacterial richness and diversity, declined the relative abundance of Lachnospiraceae_NK4A136, Ruminiclostridium and Roseburia, and increased the relative abundances of Bacteroides and Alloprevotella genera. Analysis of microbial community-level phenotypes revealed significant differences in nine phenotypes (aerobic, anaerobic, containing mobile elements, facultatively anaerobic, biofilm forming, gram-negative, gram-positive, potentially pathogenic, and stress tolerant) between CP group and CT group.Conclusions: This study indicated that mice with CP had a distinct microbiota profile.