Near infrared fluorescent probe for in vivo bioimaging of endogenous hypochlorous acid
Near infrared fluorescent probe for in vivo bioimaging of endogenous hypochlorous acid
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用于内源性次氯酸体内生物成像的近红外荧光探针
DOI：
10.1016/j.dyepig.2021.109218
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发表时间：
2021-02-23
影响因子：
4.5
通讯作者：
Liu, Yi
中科院分区：
文献类型：
--
作者：
Qian, Xiaoli;Yu, Hui;Liu, Yi
准确检测高灵敏度和高选择性的HOCl对于阐明其在健康或疾病状态下的功能至关重要。在这里，我们通过合理的设计策略和染料筛选方法，提出了一种新的荧光HOCl探针(NFL-S)。该探针具有响应速度快、灵敏度高(LOD=35.0nM)和对高氯酸根的选择性好等特点。它已成功地应用于RAW264.7细胞产生的内源性HOCl的检测，并进一步应用于小鼠关节炎模型的氧化应激成像。因此，NFL-S探针有望成为揭示HOCl3在不同生理和病理环境中作用的生物学工具。
Accurate detection of HOCl with high sensitivity and high selectivity is essential to elucidate its functions in health or disease states. Herein we propose a new fluorescence HOCl probe (NFL-S) by combining reasonable design strategies and dye screening methods. The probe NFL-S has fast response rate, excellent sensitivity (LOD = 35.0 nM) and outstanding selectivity toward HOCl. It has been successfully applied to detect endogenous HOCl produced by RAW264.7 cells, and further applied to oxidative stress imaging in a mouse arthritis model. Therefore, the probe NFL-S is highly promising to be useful for biological tool to disclose the roles of HOCl in different physiological and pathological contexts.