Stress Gene Expression in Crassostrea gigas (Thunberg, 1793) in response to experimental exposure to the toxic dinoflagellate Prorocentrum lima (Ehrenberg) Dodge, 1975
Stress Gene Expression in Crassostrea gigas (Thunberg, 1793) in response to experimental exposure to the toxic dinoflagellate Prorocentrum lima (Ehrenberg) Dodge, 1975
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DOI：
10.1111/are.12100
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发表时间：
2014-08-01
影响因子：
2
通讯作者：
Yolanda Hernandez-Saavedra, Norma
中科院分区：
文献类型：
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作者：
de Jesus Romero-Geraldo, Reyna;Yolanda Hernandez-Saavedra, Norma
有害藻华是一种对水环境有不同影响的自然现象，对人类的经济和健康都有影响。已经进行了几项基因组研究来表征污染物对太平洋牡蛎的影响。然而，牡蛎对有害藻酸盐暴露的分子反应需要更多的研究。在这项研究中，我们挑战了C。gigas与Prorocentrum利马，一种寄生毒素生产者，在受控的实验条件下，考虑甲藻密度和暴露时间（急性和亚慢性）作为变量。半定量RT-PCR分析了6个应激反应基因的表达谱：谷氨酰胺合成酶（GS）、谷氨酰胺转硫酶（GST）、热休克蛋白70（HSP 70）、热休克蛋白90（HSP 90）、铜锌超氧化物歧化酶（SOD）和黑素过氧化物酶（POX）。结果表明，这些基因的表达依赖于暴露时间和细胞浓度。一般而言，在所有测试的细胞密度中观察到表达增加，作为对暴露（0-3 h）的立即响应;而在6 h后观察到比对照低的表达水平。暴露336 h后，用3，000个细胞mL（-1）处理促进了大多数基因的表达高于对照。这是首次从分子水平上对C. gigas对甲藻水华的反应。
Harmful algal blooms (HABs) are natural phenomena with different effects on the aquatic environment that affect both human economy and health. Several genomic studies have been done to characterize the effects of contaminants on Crassostrea gigas. However, oysters' molecular response to HABs exposure needs to be studied more. In this study, we challenged C. gigas with Prorocentrum lima, a diarrhoetic toxin producer, under controlled experimental conditions considering dinoflagellate density and exposure time (acute and sub-chronic) as variables. The expression profile of six stress response genes was analysed by semiquantitative RT-PCR: Glutamine synthetase (GS), Glutathione S-transferase (GST), heat shock protein 70 (HSP70), and heat shock protein 90 (HSP90), CuZn superoxide dismutase (SOD) and melanogenic peroxidase (POX). The results revealed that these gene expressions depend on exposure time and cell concentration. In general, an increased expression was observed in all tested cell densities as immediate response to exposure (0-3 h); whereas lower expression levels than control were observed after 6 h. Treatment with 3,000 cells mL(-1) promoted higher expression than control on most genes after 336 h of exposure. This is the first report providing information at molecular level on C. gigas response to dinoflagellate blooms.