Transcriptome analysis of immune response against Vibrio harveyi infection in orange-spotted grouper (Epinephelus coioides)
Transcriptome analysis of immune response against Vibrio harveyi infection in orange-spotted grouper (Epinephelus coioides)
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DOI：
10.1016/j.fsi.2017.09.052
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发表时间：
2017-11-01
影响因子：
4.7
通讯作者：
Chen, Shih-Chu
中科院分区：
文献类型：
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作者：
Maekawa, Shun;Byadgi, Omkar;Chen, Shih-Chu
哈维氏弧菌是一种革兰氏阴性细菌，在许多水产养殖物种中都有发现。为了增加对哈维氏弧菌免疫应答的认识，本研究利用Illumina测序平台对斜带石斑鱼(Epinephelus Coioides)感染后1天和2天的头肾和脾进行了转录组分析。重新组装后，共检测到79,128个非基因，其N50为2511个碱基。在与主要数据库(NT、NR、Swiss-Prot COG、KEGG、InterPro和GO)中记录的序列比对后，根据序列相似性，可使用至少一个数据库对Unigene总数的61,208(77.4%)进行注释。哈维氏弧菌与对照组在各时间点的基因表达水平比较发现差异表达基因(Degs)(P<；0.05)：在感染后1天，头肾共获得7918个基因(上调基因5536个，下调基因2282个)，感染2天头肾共有4260个基因(上调基因1444个，下调基因2816个)，感染后1天的脾共表达7887个基因(上调基因4892个，下调基因2995个)，而感染2天的脾共表达基因8952个(上调基因7388个，下调基因1564个)。根据KEGG数据库，这些DEG主要被注释为信号转导和免疫系统类别。DEGS富含免疫相关途径功能、NOD样受体信号通路、Toll样受体信号通路、NF-kappaB信号通路和Jak-STAT信号通路。此外，我们选择了几个DEG，并通过RT-qPCR验证了它们的表达水平。这项研究的数据可能为进一步的免疫反应研究提供有价值的资源，并为防治硬骨鱼感染哈维氏弧菌提供改进的策略。(C)2017爱思唯尔有限公司。保留所有权利。
Vibrio harveyi is a gram-negative bacterium reported as found in many aquaculture species. To increase knowledge of the immune response against V. harveyi, in this study we performed transcriptome analysis of head kidney and spleen in orange-spotted grouper (Epinephelus coioides) at 1 and 2 days post-infection (dpi), using the Illumina sequencing platform. After de novo assembly, a total of 79,128 unigenes was detected with an N50 of 2511 bp. After alignments with sequences recorded in the major databases (NT, NR, Swiss-Prot COG, KEGG, Interpro and GO), based on sequence similarity, 61,208 (77.4%) of the unigene total could be annotated using at least one database. Comparison of gene expression levels between V. harveyi and a control group at each time point revealed differentially expressed genes (DEGs) (P < 0.05): a total of 7918 (5536 upregulated and 2282 downregulated genes) from head kidney at 1 day post infection (dpi), 4260 (1444 upregulated and 2816 downregulated genes) from head kidney at 2 dpi, 7887 (4892 upregulated and 2995 downregulated genes) from spleen at 1 dpi, and 8952 (7388 upregulated and 1564 downregulated genes) from spleen at 2 dpi. The DEGs were mainly annotated into signal transduction and immune system categories, based on the KEGG database. The DEGs were enriched in immune-related pathway functions, NOD-like receptor signaling pathways, Toll-like receptor signaling pathways, NF-kappa B signaling pathways, and jak-STAT signaling pathways. Additionally, we selected several DEGs and validated their expression level by RT-qPCR. The data generated in this study may provide a valuable resource for further immune response research and offer improved strategies against V. harveyi infection in teleost fishes. (C) 2017 Elsevier Ltd. All rights reserved.