Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains
Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains
复制标题
全球猪流行性腹泻病毒株的进化和基因型分析
DOI：
10.1111/tbed.12991
复制
发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Xiao，Shaobo
中科院分区：
文献类型：
--
作者：
Guo，Jiahui;Fang，Liurong;Xiao，Shaobo
猪流行性腹泻病毒（PEDV）于2010年10月在中国重新出现，目前已在全球迅速传播。对不同PEDV毒株的完整基因组进行详细分析对于了解全球重新出现的毒株和历史毒株之间的关系至关重要。在这里，我们分析了来自不同国家的409种菌株的完整基因组，这些菌株被分为五个亚组菌株（即，GI-a、GI-B、GII-a、GII-B和GII-c）。对PEDV毒株中不同基因的系统发育研究表明，新发现的亚群GII‐c在刺突基因和其他基因之间表现出不一致的拓扑结构。此外，重组分析表明，GII-c病毒是从重组病毒进化而来的，该重组病毒获得了来自GII-a亚组的刺突基因的5′部分和来自GII-a亚组的其余基因组区域。分子钟分析显示，GII-c亚组刺突基因的分歧发生在2010年4月，表明该亚组起源于PEDV重新出现爆发之前的重组事件。有趣的是，猪蛔虫（一种发生在猪身上的大型蛔虫）被发现是一种不寻常的PEDV宿主，为跨宿主传播提供了潜在的支持。这项研究对了解正在进行的全球PEDV爆发具有重要意义，并将指导未来的努力，以制定有效的预防措施，对PEDV。
Porcine epidemic diarrhea virus (PEDV), which re‐emerged in China in October 2010, has spread rapidly worldwide. Detailed analyses of the complete genomes of different PEDV strains are essential to understand the relationships among re‐emerging and historic strains worldwide. Here, we analysed the complete genomes of 409 strains from different countries, which were classified into five subgroup strains (i.e., GI‐a, GI‐b, GII‐a, GII‐b, and GII‐c). Phylogenetic study of different genes in the PEDV strains revealed that the newly discovered subgroup GII‐c exhibited inconsistent topologies between the spike gene and other genes. Furthermore, recombination analysis indicated that GII‐c viruses evolved from a recombinant virus that acquired the 5′ part of the spike gene from the GI‐a subgroup and the remaining genomic regions from the GII‐a subgroup. Molecular clock analysis showed that divergence of the GII‐c subgroup spike gene occurred in April 2010, suggesting that the subgroup originated from recombination events before the PEDV re‐emergence outbreaks. Interestingly,Ascaris suum, a large roundworm occurring in pigs, was found to be an unusual PEDV host, providing potential support for cross‐host transmission. This study has significant implications for understanding ongoing global PEDV outbreaks and will guide future efforts to develop effective preventative measures against PEDV.