Biological variation of free plasma amino acids in healthy individuals
Biological variation of free plasma amino acids in healthy individuals
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DOI：
10.1515/cclm.2010.008
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发表时间：
2010-01-01
影响因子：
6.8
通讯作者：
Venta, Rafael
中科院分区：
文献类型：
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作者：
Corte, Zoraida;Venta, Rafael
背景：更全面的数据库缺乏游离血浆氨基酸的生物学变异数据。因此，我们确定了健康受试者的个体内和个体间变异成分。这些数据被用来定义理想的目标不精确，偏差和总误差，指数的个性和参考变化values.Methods：血浆样本收集11名志愿者在每周间隔超过5周。游离血浆氨基酸进行了分析，通过反相高效液相色谱法和分析和生物变异数据，来自使用AN0VA.Results：个体内变异系数范围从9.5%到46.4%，与较低的值之间的必需氨基酸。平均个体间变异系数为46.6%，高于所有氨基酸的个体内值。因此，个性指数低于0.8。参考值变化范围为30.9%~128.4%，总误差范围为15.2%~61.0%。结论：血浆氨基酸的个体内变异系数较低，必需氨基酸的稳态控制较强。分析质量目标可以合理地实现与当前的方法。参考区间在检测个体异常结果方面的价值有限。因此，建议通过参考变化值对系列结果进行比较。临床化学实验室医学2010; 48：99 - 104。
Background: Biological variation data for free plasma amino acids are lacking from the more comprehensive databases. Therefore, we determined the intra-and inter-individual components of variation in healthy subjects. These data were used to define desirable goals for imprecision, bias and total error, indices of individuality and reference change values.Methods: Plasma samples were collected from 11 volunteers at weekly intervals over 5 weeks. Free plasma amino acids were analyzed by reversed-phase HPLC and analytical and biological variation data were derived using ANOVA.Results: Intra-individual coefficients of variation ranged from 9.5% to 46.4%, with lower values among the essential amino acids. The mean inter-individual coefficient of variation was 46.6% and was higher than intra-individual values for all amino acids. Thus, indices of individuality were below 0.8. Reference change values ranged from 30.9% to 128.4% and total error values ranged from 15.2% to 61.0%.Conclusions: Plasma amino acids exhibit relatively low intra-individual coefficients of variation, with essential amino acids showing tighter homeostatic control. Analytical quality goals can be reasonably achieved with current methods. Reference intervals are of limited value in the detection of unusual results in an individual. Therefore, comparison of serial results by means of the reference change values is recommended. Clin Chem Lab Med 2010; 48: 99-104.