CENTROSOME BEHAVIOR UNDER THE ACTION OF A MITOCHONDRIAL UNCOUPLER AND THE EFFECT OF DISRUPTION OF CYTOSKELETON ELEMENTS ON THE UNCOUPLER-INDUCED ALTERATIONS
CENTROSOME BEHAVIOR UNDER THE ACTION OF A MITOCHONDRIAL UNCOUPLER AND THE EFFECT OF DISRUPTION OF CYTOSKELETON ELEMENTS ON THE UNCOUPLER-INDUCED ALTERATIONS
复制标题
DOI：
10.1006/jsbi.1994.1056
复制
发表时间：
1994-11-01
影响因子：
3
通讯作者：
VOROBJEV, IA
中科院分区：
文献类型：
--
作者：
ALIEVA, IB;VOROBJEV, IA
羰基氰化物对（三氟甲氧基）苯腙（FCCP）诱导猪肾胚胎细胞线粒体在2 ~ 3 min内失去罗丹明123染色。罗丹明123染色细胞接种FCCP后5min内，细胞质无弥漫性染色。FCCP没有引起细胞质微管复合物的改变，但引起母体中心粒的非随机（优先垂直于底物表面）取向。母体中心粒在治疗后10分钟出现非随机取向，并持续2小时。在用药后30分钟，FCCP治疗增加了母体中心粒上的平均中心粒周围卫星数和初级纤毛的频率。FCCP处理诱导的垂直于底物的中心粒百分比因微管的破坏而略有增加，因微丝的破坏而略有减少。在两种情况下，中心粒的取向与随机有显著差异（P < 0.01）。这些结果表明，微管既不参与从质膜到中心粒的信号通路，也不像Albrecht-Buehler和Bushnell （Experimental Cell Research 120,1979）提出的那样，将中心粒垂直于底物锚定。(C) 1994学术出版社，Inc。
Carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) induced in pig kidney embryo cells a loss of rhodamine 123 staining of mitochondria in 2-3 min. Within 5 min after FCCP inoculation of cells prestained with rhodamine 123, the diffuse staining of the cytoplasm was absent. FCCP did not induce changes in the cytoplasmic microtubule complex, but induced nonrandom (preferentially perpendicular to the substrate surface) orientation of maternal centrioles. Nonrandom orientation of maternal centrioles occurred 10 min after treatment and remained for 2 hr. At 30 min after introduction of the drug, FCCP treatment increased the mean number of pericentriolar satellites on maternal centrioles and the frequency of primary cilia. The percentage of centrioles perpendicular to the substrate induced by FCCP treatment was slightly increased by disruption of microtubules and slightly diminished by disruption of microfilaments. In both cases centrioles were oriented significantly differently from random (P < 0.01). These results suggest that microtubules are neither involved in the signaling pathway from plasma membrane to the centriole, nor do they anchor the centrioles perpendicular to the substrate, as proposed by Albrecht-Buehler and Bushnell (Experimental Cell Research 120, 1979). (C) 1994 academic Press, Inc.