ORGANIZATION OF THE SEPTAL REGION IN THE RAT-BRAIN - A GOLGI EM STUDY OF LATERAL SEPTAL NEURONS
ORGANIZATION OF THE SEPTAL REGION IN THE RAT-BRAIN - A GOLGI EM STUDY OF LATERAL SEPTAL NEURONS
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DOI：
10.1002/cne.902860406
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发表时间：
1989-08-22
影响因子：
2.5
通讯作者：
FROTSCHER, M
中科院分区：
文献类型：
--
作者：
ALONSO, JR;FROTSCHER, M
应用高尔基体/电镜（Golgi/EM）联合技术对大鼠外侧隔（LS）内不同类型神经元的精细结构和突触组织进行了分析，在隔复合体的背外侧（LSd）、中间外侧（LSi）和腹外侧（LSv）核中。两种特征性的细胞类型中观察到的LSd：I型厚，短树突密集覆盖短刺，和II型与较长和较薄的树突表现出更少，但更长的刺。后一种类型是迄今为止最频繁浸渍的细胞类型在LSd，也存在于LSi。任一细胞类型的棘上的突触接触是不对称的，大多数的突触前扣含有明确的圆形突触囊泡。偶尔终端被发现，同时包含明确和致密的核心囊泡。LSi内可见典型的梭形神经元，棘数少，树突较长，有时侵入LS的其他核团。LSv含有大量的小神经元树突状野。一个相对较大数量的终端与致密核心囊泡被发现建立突触接触，确定LSV神经元。LS神经元的形态异质性进行了讨论，关于传入和传出纤维系统的其他研究，以及免疫组化研究的这个特定区域的隔复合体。
The combined Golgi/electron microscope (EM) technique was used to analyze the fine structure and synaptic organization of the various types of neurons in the rat lateral septum (LS), i.e., in the dorsolateral (LSd), intermediolateral (LSi), and ventrolateral (LSv) nuclei of the septal complex. Two characteristic cell types were observed in the LSd: type I with thick, short dendrites densely covered with short spines, and type II with longer and thinner dendrites exhibiting fewer but longer spines. This latter type was by far the most frequently impregnated cell type in the LSd and was also present in the LSi. Synaptic contacts on spines of either cell type were asymmetric; the majority of the presynaptic boutons contained clear round synaptic vesicles. Occasionally terminals were found that contained both clear and dense-core vesicles. Typical fusiform neurons with a low number of spines and rather long dendrites, sometimes invading other LS nuclei, were found in the LSi. The LSv contained numerous small neurons dendritic fields. A relatively larger number of terminals with dense-core vesicles were found to establish synaptic contacts with identified LSv neurons. The morphological heterogeneity of LS neurons is discussed with regard to other studies on afferent and efferent fiber systems as well as immunohistochemical studies of this particular region of the septal complex.