Mild hypothermia provides significant protection against ischemia/reperfusion injury in livers of obese and lean rats
Mild hypothermia provides significant protection against ischemia/reperfusion injury in livers of obese and lean rats
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DOI：
10.1097/01.sla.0000154259.73060.f1
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发表时间：
2005-03-01
影响因子：
9
通讯作者：
Nienmann, CU
中科院分区：
文献类型：
--
作者：
Choi, S;Noh, J;Nienmann, CU
目的：评估麻醉诱导的轻度全身低温对瘦和肥胖大鼠在热ischemia.Summary背景数据：肝热缺血在手术过程中仍然是一个重要的问题，特别是在假定的基线dysfunction.Methods：左和中叶的男性瘦和肥胖Zucker大鼠暴露于75分钟的缺血下，无论是轻度低温或常温条件。缺血75分钟后。再灌注器官并观察动物24小时。存活的动物被杀死，血液和组织收获，以确定肝酶和检查histologics.Results：亚低温显着降低肝细胞损伤瘦和肥胖大鼠。低温组肝损伤生化指标明显降低（P < 0.01）。在再灌注24小时时，常温和低温瘦型组的存活率没有差异。然而，与常温肥胖大鼠相比，低温显著增加了肥胖大鼠的存活率（100%对20%，P < 0.01）。与低温动物相比，在经历超过75%坏死的常温瘦型和肥胖动物中坏死更明显。与此相反，轻度低温可使瘦大鼠的肝坏死率低于25%，肥胖大鼠的肝坏死率低于50%。结论：在临床相关模型中，我们证实轻度低温可显著降低瘦大鼠和肥胖大鼠热缺血模型中的肝损伤，并显著提高肥胖大鼠的24小时存活率。
Objective: To evaluate the effect of anesthesia induced mild systemic hypothermia on hepatic injury in lean and obese rats during warm ischemia.Summary Background Data: Hepatic warm ischemia during surgery remains a significant problem, particularly in organs with presumed baseline dysfunction.Methods: The left and median lobes of male lean and obese Zucker rats were exposed to 75 minutes of ischemia under either mild hypothermic or normothermic conditions. After 75 minutes of ischemia. the organs were reperfused and animals were observed for 24 hours. Surviving animals were killed and blood and tissue was harvested to determine liver enzymes and examine the histology.Results: Mild hypothermia significantly decreased hepatocelular injury in both lean and obese rats. Biochemical markers of hepatic injury were significantly reduced in hypothermic groups (P < 0.01). Survival in normo- and hypothermic lean groups were not different at 24 hours of reperfusion. However, hypothermia profoundly increased survival in obese rats when compared with normothermic obese rats (100% versus 20%, P < 0.01). Necrosis was more pronounced in both normothermic lean and obese animals who experienced more than >75% necrosis when compared with hypothermic animals. In contrast, mild hypothermia reduced necrosis in lean rats to less than 25% and in obese rats to less than 50%.Conclusions: We demonstrated in a clinically relevant model that mild hypothermia significantly reduces hepatic injury in a warm ischemia model in lean and obese rats and significantly improved 24-hour survival in obese rats.