Online monitoring of oxygen in spinner flasks
Online monitoring of oxygen in spinner flasks
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DOI：
10.1007/s10529-009-9919-2
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发表时间：
2009-05-01
影响因子：
2.7
通讯作者：
Heinzle, Elmar
中科院分区：
文献类型：
--
作者：
Deshpande, Rahul Ravi;Heinzle, Elmar
我们展示了一种新型光学在线传感器的应用，该传感器固定在旋转烧瓶中，用于在线监测哺乳动物细胞生长过程中溶解的O-2浓度。使用这种传感器，只需对烧瓶进行微小的改变，我们就可以确定体积O-2传递系数以及O-2消耗率。在正常生长条件下，细胞不受O-2限制。此外，O-2从大气到旋转烧瓶的转移也受到螺旋盖使用的影响。在线测量进一步应用于确定O-2吸收率，然后可用于监测细胞的代谢状态，也用于在线过程监测。
We show the application of a novel optical on-line sensor fixed in spinner flasks for the online monitoring of dissolved O-2 concentrations during mammalian cell growth. Using this sensor that requires only minute changes to the flask to be made, we could determine the volumetric O-2 transfer coefficient as well as O-2 consumption rates. Under normal growth conditions the cells did not undergo O-2 limitation. Also, the transfer of O-2 from the atmosphere to the spinner flasks is influenced by the use of screw caps. The on-line measurement was further applied to determine the O-2 uptake rates which can then be used to monitor the metabolic state of the cells and also for online process monitoring.