Paraffin section detection of the c-kit gene product (CD117) in human tissues:: Value in the diagnosis of mast cell disorders
Paraffin section detection of the c-kit gene product (CD117) in human tissues:: Value in the diagnosis of mast cell disorders
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DOI：
10.1016/s0046-8177(98)90066-1
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发表时间：
1998-05-01
期刊：
影响因子：
3.3
通讯作者：
Weiss, LM
中科院分区：
文献类型：
--
作者：
Arber, DA;Tamayo, R;Weiss, LM
已知c-hit基因产物（CD117）在多种正常人类组织细胞类型中表达，包括乳腺上皮细胞、生殖细胞、黑素细胞、未成熟骨髓细胞和肥大细胞。为了进一步表征该抗原的表达，我们对117个正常人体组织和576个肿瘤组织进行了石蜡切片免疫组化研究。在各种正常细胞和53%的肿瘤中发现了不同程度的CD117表达。在大多数病例中（占总数的42%），CD117表达较弱。最常见于肥大细胞病（100%）、睾丸生殖细胞瘤（100%）、子宫内膜癌（100%）、甲状腺乳头状和滤泡性癌（100%）、小细胞癌（91%）、恶性黑色素瘤（90%）和卵巢上皮癌（87%）。强免疫反应性仅在肥大细胞病（11例中的11例）、浆液性卵巢癌（16例中的3例）、恶性黑色素瘤（40例中的2例）、小细胞肺癌（7例中的1例）和腺样囊性癌（1例中的1例）中被发现。虽然反应性的模式主要是细胞质的，但在一小部分病例中发现了膜染色模式，在正常肥大细胞和所有肥大细胞疾病病例中发现了强烈的膜染色。该抗原弱表达缺乏肿瘤特异性，限制了其在大多数病例中的诊断效用。然而，在肥大细胞中发现的CD117的强膜反应性可能对肥大细胞疾病的诊断有用。版权所有(C) 1998由W.B. Saunders公司。
The c-hit gene product (CD117) is known to be expressed by a variety of normal human tissue cell types, including breast epithelium, germ cells, melanocytes, immature myeloid cells, and mast cells. To further characterize the expression of this antigen, 117 normal human tissues and 576 human tumors were studied by paraffin section immunohistochemistry. Varying degrees of CD117 expression were identified in various normal cells and in 53% of all tumors studied. In most cases (42% of total), CD117 expression was weak. Expression was most common in mast cell disease (100%), testicular germ cell tumors (100%), endometrial carcinomas (100%), papillary and follicular thyroid carcinomas (100%), small cell carcinomas (91%), malignant melanomas (90%), and ovarian epithelial carcinomas (87%). Strong immunoreactivity was only identified in cases of mast cell disease (11 of 11 cases), serous ovarian carcinoma (3 of 16), malignant melanoma (2 of 40), small cell lung carcinoma (one of seven), and adenoid cystic carcinoma (one of one). Although the pattern of reactivity was primarily cytoplasmic, a membrane staining pattern was seen in a subset of cases, and strong membrane staining was identified in normal mast cells and all cases of mast cell disease. The lack of tumor specificity of weak expression of this antigen limits its diagnostic utility in most cases. However, the strong membrane reactivity for CD117 identified in mast cells may be useful in the diagnosis of mast cell disorders. Copyright (C) 1998 by W.B. Saunders Company.