Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating Hepcidin
Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating Hepcidin
复制标题
补铁通过调节铁调素减轻贫血仔猪的炎症状态
DOI：
10.1007/s12011-015-0295-6
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发表时间：
2015-09-01
影响因子：
3.9
通讯作者：
Du, Huahua
中科院分区：
文献类型：
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作者：
Pu, Yutian;Guo, Bingxiu;Du, Huahua
缺铁在世界各地都很常见，并与免疫力受损有关。我们用小猪来模拟人类婴儿，评估全身铁稳态对促炎状态的影响。在3日龄时，通过肌内给药，向动物饲养的仔猪胃肠外供应右旋糖酐铁。相对于没有铁补充（对照），铁葡聚糖治疗（FeDex）仔猪增加血液学参数以及铁水平的血清和组织从第21天至第49天。铁调素在FeDex处理的仔猪中观察到高表达，这与十二指肠中铁转运蛋白的表达抑制相关。在FeDex处理的仔猪回肠中检测到较低水平的促炎细胞因子（IL-6、TNF-α、IFN-γ和IL-1 β）转录物，这表明补铁可以减轻铁缺乏引起的炎性细胞因子的增加。肝脏和十二指肠的组织病理学分析证明补铁后炎症反应减轻。铁调素在FeDex的刺激下具有明显的抗炎作用，提示铁调素可能具有抗炎作用，是铁调节与炎症之间的一个候选调节因子。
Iron deficiency is common throughout the world and has been linked to immunity impairments. Using piglets to model human infants, we assessed the impact of systemic iron homeostasis on proinflammatory status. Artificially reared piglets were parenterally supplied with iron dextran by intramuscular administration at the age of 3 days. Relative to no iron supplementation (control), iron dextran-treated (FeDex) piglets increased hematological parameters as well as iron levels in serum and tissues from days 21 to 49. High expression of hepcidin was observed in FeDex-treated piglets, which correlated with suppressed expression of ferroportin in duodenum. Lower levels of proinflammatory cytokine (IL-6, TNF-alpha, IFN-gamma, and IL-1 beta) transcripts were detected in ileum of FeDex-treated piglets, which indicated that iron supplementation could attenuate the increase of inflammatory cytokines caused by iron deficiency. Histopathological analysis of liver and duodenum proved the less inflammatory responses after iron supplementation. Hepcidin was highly stimulated by FeDex supplementation and attenuated the inflammation of anemia, which implied that hepcidin might had antiinflammatory function and is a candidate regulator of the cross-talk between iron regulation and inflammation.