Characterization of a new animal model of chronic hyperprolactinemia.
Characterization of a new animal model of chronic hyperprolactinemia.
复制标题
慢性高催乳素血症新动物模型的表征。
DOI：
10.1016/0026-0495(91)90111-9
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
MacLeod,RM
中科院分区：
文献类型：
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作者：
Adler,RA;Krieg,RJ;Farrell,ME;Deiss,WP;MacLeod,RM
慢性催乳素(PRL)过多的动物模型包括携带可移植垂体肿瘤的大鼠，这些肿瘤除了PRL外还产生其他垂体激素。我们报告了一种新的模型的特征，水牛大鼠植入了MMQ肿瘤，一种从7315A系发展而来的系。植入MMQ肿瘤的大鼠的血清PRL水平随着时间的推移而增加，并与皮下肿瘤的估计体积相关。当植入4周后处死大鼠时，荷瘤大鼠的血清PRL水平显著高于对照组(雌性，2,723±266vs192±46 ng/m L，P<；0.0001；雄性，1,637±213v99±11 ng/m L，P<；.0001)。在荷瘤和对照的水牛大鼠中，Nb2淋巴瘤测定的血清PRL水平都高于免疫测定。对荷瘤大鼠血清的葡聚糖凝胶层析表明，大部分PRL免疫反应性与125I-rPRL共洗脱。荷瘤大鼠血清生长激素(GH)和黄体生成素(LH)水平与对照组均无差异。携带MMQ的雌性大鼠雌二醇水平较低。死亡时，肾上腺、肾脏和性腺的湿重不受肿瘤存在的影响。相反，荷瘤大鼠的脾重量和白髓增生的组织学证据有所增加。水牛移植PRL-MMQ肿瘤是研究慢性高催乳素血症的一种有前景的新工具。
Animal models of chronic prolactin (PRL) excess have included rats bearing transplantable pituitary tumors that have produced other hypophyseal hormones in addition to PRL. We report characterization of a new model, the Buffalo rat implanted with the MMQ tumor, a line developed from the 7315a line. Rats implanted with the MMQ tumor have serum PRL levels that increase with time and correlate with the estimated volume of the subcutaneous tumor. When rats are killed 4 weeks after implantation, serum PRL levels are strikingly higher in tumor-bearing rats compared with controls (females, 2,723 ± 266 v 192 ± 46 ng/mL,P< .0001; males, 1,637 ± 213v99 ± 11 ng/mL,P< .0001). Serum PRL levels measured by the Nb2lymphoma assay were higher than immunoassay measurements in both tumor-bearing and control Buffalo rats. Sephadex chromatography of serum from tumor-bearing rats showed that most of the PRL immunoreactivity co-eluted with125I-rPRL. Neither serum growth hormone (GH) nor luteinizing hormone (LH) levels were different from controls in tumor-bearing rats. Female MMQ-bearing rats had lower estradiol levels. At death, the wet weights of adrenal glands, kidneys, and gonads were not affected by the presence of tumor. In contrast, tumor-bearing rats had increased spleen weight and histological evidence of white pulp hyperplasia. The Buffalo rat implanted with the PRL-only MMQ tumor is a promising new tool for the study of chronic hyperprolactinemia.