Correlation between clinical activity of systemic lupus erythematosus and the amounts of DNA in DNA/anti-DNA antibody immune complexes.
Correlation between clinical activity of systemic lupus erythematosus and the amounts of DNA in DNA/anti-DNA antibody immune complexes.
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系统性红斑狼疮的临床活动与 DNA/抗 DNA 抗体免疫复合物中 DNA 量之间的相关性。
DOI：
10.4049/jimmunol.129.5.1960
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发表时间：
1982
影响因子：
4.4
通讯作者：
A. Steinberg
中科院分区：
文献类型：
--
作者：
C. Morimoto;H. Sano;T. Abe;M. Homma;A. Steinberg
研究了系统性红斑狼疮 (SLE) 的临床活动与从 DNA/抗 DNA 抗体免疫复合物中分离的 DNA 片段的分子大小之间的关系。在检查的 28 名 SLE 患者的血清中，鉴定出三种不同分子大小的 DNA，即 m.v. 的 DNA。 25,000 个，含 30 至 50 个碱基对 (bp)；分子量100,000 个，150 至 200 bp；和分子量200,000 个，300 bp。根据DNA片段的分子大小，我们可以将SLE患者分为四组。第一组含有主要为分子量的 DNA。 25,000。第二组含有主要为分子量的 DNA。 100,000。第三组含有 m.w. 的 DNA。 200,000。第四组包含 m.w. 25,000 和 100,000。开发了一种定量 DNA/抗 DNA 免疫复合物中 DNA 量的方法。通过测量掺入 DNA 5 端的 32P-磷酸盐的量来估计 DNA 片段的量。患有严重疾病的患者往往有更多的 DNA（高达 400 ng/ml 血清）。两项系列研究也支持这一结果。因此，定量分析表明，免疫复合物中的 DNA 量与疾病活动度高度相关（r = 0.864；p 小于 0.001）。这些结果表明DNA/抗DNA免疫复合物可能在狼疮性肾炎的发病机制中发挥一定作用。
The relationship between clinical activity of systemic lupus erythematosus (SLE) and molecular sizes of DNA fragments isolated from DNA/anti-DNA antibody immune complexes were examined. Among sera from twenty-eight patients with SLE examined, three different molecular sizes of DNA were identified, namely, DNA of m.v. 25,000 with 30 to 50 base pairs (bp); m.w. 100,000 with 150 to 200 bp; and m.w. 200,000 with 300 bp. On the basis of the molecular sizes of DNA fragments, we can divide patients with SLE into four groups. The first group contained DNA predominantly of m.w. 25,000. The second group contained DNA predominantly of m.w. 100,000. The third group contained DNA of m.w. 200,000. The fourth group contained both m.w. 25,000 and 100,000. A method to quantitate the amounts of DNA in DNA/anti-DNA immune complexes was developed. The amount of DNA fragments was estimated by measuring the amount of 32P-phosphate incorporated into 5 ends of DNA. Patients with severe disease tended to have greater amounts of DNA (up to 400 ng/ml serum). Two serial studies also support this result. Thus, the quantitative analysis shows that the amount of DNA in the immune complexes is highly correlated with disease activity (r = 0.864; p less than 0.001). These results suggest that DNA/anti-DNA immune complexes may play some role in the pathogenesis of lupus nephritis.