Evidence that contact with connective tissue matrix is required for normal interaction between Schwann cells and nerve fibers.
Evidence that contact with connective tissue matrix is required for normal interaction between Schwann cells and nerve fibers.
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DOI：
10.1083/jcb.78.3.943
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发表时间：
1978-09
影响因子：
7.8
通讯作者：
Bunge, M B
中科院分区：
文献类型：
--
作者：
Bunge, R P;Bunge, M B
在没有成纤维细胞的情况下，在轴突和雪旺细胞生长的条件下，胚胎大鼠感觉神经节的外植体偶尔会发育出神经束，这些神经束部分悬浮在胶原底物上方的培养基中。在这些悬浮区，神经束是异常的，雪旺细胞数量减少，局限于沿神经束偶尔聚集，仅为神经束周围的少数轴突提供鞘层，并且不形成髓磷脂。当这些神经束以重组大鼠尾胶原蛋白为底物时，雪旺细胞数量增加，小神经纤维正常形成鞘鞘，较大的轴突有髓鞘。我们得出结论，对于正常发育，雪旺细胞需要与细胞外基质和轴突接触。悬吊神经束中的雪旺细胞异常与营养不良小鼠的神经根相似。
Explants of fetal rat sensory ganglia, cultured under conditions allowing axon and Schwann cell outgrowth in the absence of fibroblasts, occasionally develop nerve fascicles that are partially suspended in culture medium above the collagen substrate. In these suspended regions, fascicles are abnormal in that Schwann cells are decreased in number, are confined to occasional clusters along the fascicle, provide ensheathment for only a few axons at the fascicle periphery, and do not form myelin. When these fascicles are presented with a substrate of reconstituted rat-tail collagen, Schwann cell numbers increase, ensheathment of small nerve fibers occurs normally, and larger axons are myelinated. We conclude that, for normal development, Schwann cells require contact with extracellular matrix as well as axons. The Schwann cell abnormalities in suspended fascicles are similar to those observed in nerve roots of dystrophic mice.