COMBINED H-1 AND P-31 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPIC STUDIES OF BICUCULLINE-INDUCED SEIZURES INVIVO
COMBINED H-1 AND P-31 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPIC STUDIES OF BICUCULLINE-INDUCED SEIZURES INVIVO
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DOI：
10.1002/ana.410200203
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发表时间：
1986-08-01
影响因子：
11.2
通讯作者：
SHULMAN, RG
中科院分区：
文献类型：
--
作者：
PETROFF, OAC;PRICHARD, JW;SHULMAN, RG
用1.89特斯拉(Tesla)光谱仪，对用30%氧化亚氮中的氧气换气的瘫痪兔脑进行了1H和31P核磁共振波谱测定。用核磁共振方法监测荷包牡丹碱致痫过程中和发作后脑细胞内pH值和乳酸浓度的变化，以及脑电、心率和动脉血压的变化。在持续一个多小时的癫痫发作中，脑细胞内pH变得酸性，脑组织乳酸水平迅速上升，这两种变化都持续了长达2小时，没有恢复的迹象。在较短时间的癫痫发作后，尽管细胞内pH和脑电几乎完全恢复，乳酸升高的持续时间并没有减少。
Using a 1.89-Tesla spectrometer, 1H and 31P nuclear magnetic resonance spectra were acquired from the brains of paralyzed rabbits ventilated with 30% oxygen in nitrous oxide. Intracellular pH and changes in lactate concentration in the cerebrum were monitored by nuclear magnetic resonance methods during and after bicuculline-induced seizures, together with the electroencephalogram, heart rate, and arterial blood pressure. During seizures lasting more than an hour, cerebral intracellular pH became acidic, the cerebral lactate level rose rapidly, and both changes persisted as long as 2 hours without signs of recovery. After less prolonged seizures, lactate elevations were no less persistent, despite nearly complete recovery of intracellular pH and electroencephalogram.