B-cell subpopulations in children: National reference values.
B-cell subpopulations in children: National reference values.
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DOI：
10.1002/iid3.26
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发表时间：
2014-11
影响因子：
3.2
通讯作者：
Picard, Capucine
中科院分区：
文献类型：
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作者：
Duchamp, Marie;Sterlin, Delphine;Diabate, Aminata;Uring-Lambert, Beatrice;Guerin-El Khourouj, Valerie;Le Mauff, Brigitte;Monnier, Delphine;Malcus, Christophe;Labalette, Myriam;Picard, Capucine
外周 B 淋巴细胞在生命的最初几年经历一系列变化。与外来抗原的相遇导致成熟和分化。影响 B 细胞发育的几种一抗缺陷 (PAD) 与 B 细胞区室的组成和/或分化异常有关。最新的原发性免疫缺陷 (PID) 和常见变异免疫缺陷 (CVID) 国际分类强调了 B 细胞免疫表型分析和年龄特异性参考区间对于诊断目的的重要性。我们根据 242 名健康儿童外周血中 CD27 和表面 IgD 的表达情况，建立了记忆 B 细胞亚群的国家参考值。我们在此报告了从新生儿到成人的七个年龄组的初始、转换和非转换记忆 B 细胞的绝对计数和百分比。我们发现，幼稚 B 细胞百分比在 6 个月至 8 岁之间下降，之后稳定在 70-80% 左右。记忆 B 细胞在出生时就已经存在，并且其数量在整个童年时期不断增加，并在 12 至 18 岁之间稳定下来。儿童 B 细胞水平参考区间的定义应有助于各种 B 细胞免疫缺陷的筛查和诊断。这项多中心研究提供了国家参考值，因此应有助于儿童的免疫学诊断。
Peripheral B-lymphocytes undergo a series of changes during the first few years of life. Encounters with foreign antigens lead to maturation and differentiation. Several primary antibody deficiencies (PADs) affecting B-cell development are associated with abnormalities in the composition and/or differentiation of B-cell compartments. The most recent international classifications of primary immunodeficiencies (PIDs) and common variable immunodeficiencies (CVID) have highlighted the importance of B-cell immunophenotyping and age-specific reference intervals for diagnostic purposes. We established national reference values for memory B-cell subpopulations, on the basis of CD27 and surface IgD expression in the peripheral blood of 242 healthy children. We report here the absolute counts and percentages of naive, switched and non-switched memory B-cells for seven age groups, from neonates to adults. We found that the naive B-cells percentage declined between the ages of 6 months and 8 years, after which it remained stable at about 70–80%. Memory B-cells are already present at birth and their numbers increase throughout childhood, stabilizing between the ages of 12 and 18 years. The definition of reference intervals for pediatric B-cell levels should facilitate the screening and diagnosis of various B-cell immunodeficiencies. This multicenter study, providing national reference values, should thus facilitate immunological diagnosis in children.