Induction of matrix metalloproteinase 9 expression in breast carcinoma cells by a soluble factor from fibroblasts.
Induction of matrix metalloproteinase 9 expression in breast carcinoma cells by a soluble factor from fibroblasts.
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通过成纤维细胞的可溶性因子诱导乳腺癌细胞中基质金属蛋白酶 9 的表达。
DOI：
10.1007/bf00053892
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发表时间：
1996
影响因子：
4
通讯作者：
Muschel,RJ
中科院分区：
文献类型：
--
作者：
Himelstein,BP;Muschel,RJ
肿瘤-间质的相互作用似乎在诱导恶性肿瘤中金属蛋白酶的表达中起着重要作用。我们描述了一种组织培养系统，在该系统中，明胶酶B或92 kDa IV型胶原酶/明胶酶通过与乳腺癌细胞株共培养诱导基质金属蛋白酶9的表达。乳腺癌细胞和正常大鼠胚胎成纤维细胞在培养过程中都不能单独产生基质金属蛋白酶-9，而人基质金属蛋白酶-9是在两者共同培养时产生的。基质金属蛋白酶-9以潜伏期形式分泌。这种诱导作用至少部分是通过增加乳腺癌细胞中的基质金属蛋白酶-9mRNA水平而发生的。这些增加似乎不需要蛋白质合成。成纤维细胞的条件培养液可以复制在乳腺癌细胞系中对基质金属蛋白酶-9的诱导。培养基中的活性因子被加热或胰酶灭活，表明它是一种蛋白质。该蛋白的大小在30-100 kDa之间。因此，成纤维细胞可以分泌一种能够调节乳腺癌细胞中基质金属蛋白酶-9表达的因子。
Tumor-stromal interactions appear to play an important role in the induction of metalloproteinase expression in malignant tumors. We describe a tissue culture system in which expression of MMP-9 (gelatinase B or the 92 kDa type IV collagenase/gelatinase) was induced by co-cultivation of fibroblasts with breast cancer cell lines. While neither the breast cancer cells nor the normal rat embryo fibroblasts made MMP-9 alone in culture, human MMP-9 was made in the co-cultures. The MMP-9 was secreted in a latent form. The induction occurred at least in part through increases in the MMP-9 mRNA levels in the breast cancer cells. These increases did not appear to require protein synthesis. Conditioned medium from the fibroblasts could duplicate the induction of MMP-9 in the breast cancer cell lines. The active factor in the medium was inactivated by heat or by trypsin suggesting that it was a protein. This protein was in the size range of 30–100 kDa. Thus, fibroblasts could secrete a factor which was able to regulate the expression of MMP-9 in breast cancer cells.