Traditional Chinese medicine for the treatment of primary dysmenorrhea: How do Yuanhu painkillers effectively treat dysmenorrhea?
Traditional Chinese medicine for the treatment of primary dysmenorrhea: How do Yuanhu painkillers effectively treat dysmenorrhea?
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DOI：
10.1016/j.phymed.2013.05.003
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发表时间：
2013-09-15
期刊：
影响因子：
7.9
通讯作者：
Wang, Siwang
中科院分区：
文献类型：
--
作者：
Chen, Yuetao;Cao, Yu;Wang, Siwang
目的：目的：观察元胡止痛片（YHP）治疗原发性痛经的临床疗效，揭示其作用机理。大鼠灌胃给予0.698 g/kg YHP、0.07 g/kg延胡索乙素（THP; YHP的主要成分）、0.02 g/kg欧前胡素（IMP）或THP + IMP（0.07 + 0.02 g/kg）复方制剂（PG）。对子宫组织样本进行H&E染色和组织病理学检查。检测超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）、i-kappa B、核因子-kappa B（NF-κ B）和环氧合酶-2（考克斯-2）指数。PG对原发性痛经模型大鼠子宫收缩有明显的抑制作用（p < 0.05），与单药治疗相比有显著性差异（p < 0.05）。病理组织学检查显示对照组子宫炎症明显减轻。THP以浓度依赖性方式显著抑制Ach、PGF（2 α）和催产素引起的离体子宫收缩。THP和IMP均显著影响NO水平和NF-κ B B活化，上调i-kappa B表达，下调iNOS和考克斯-2表达。IMP能明显降低MDA含量，提高SOD活性（p < 0.05）。结论：益宫散对大鼠原发性痛经具有保护作用，能明显减轻实验性痛经的严重程度。联合用药比单用THP或IMP更有效，联合用药对原发性痛经可能有协同作用。可能解释其有益作用的机制包括减轻氧化应激，抑制过度炎症反应，以及通过抑制细胞外Ca 2+内流来减轻离体大鼠子宫的收缩。未来临床实践的广阔潜力是可以预见的。(C)2013 Elsevier GmbH. All rights reserved.
Aim: To examine the efficacy of YuanHu painkillers (YHP) as a treatment for primary dysmenorrhea and to reveal YHP's principle formula.Methods: A Wistar rat uterine contraction model was utilized in this study. Rats were given 0.698 g/kgYHP, 0.07 g/kg tetrahydropalmatine (THP; YHP's main component), 0.02 g/kg imperatorin (IMP), or THP + IMP (0.07 + 0.02 g/kg) as polypharmacy (PG) by gavage. H&E staining and histopathological examination of the uteri tissue samples were performed. We then detected superoxide dismutase (SOD) and malondialdehyde (MDA), nitric oxide (NO), as well as inducible nitric oxide synthase (iNOS), i-kappa B, nuclear factor-kappa B (NF-kappa B), and cyclooxygenase-2 (COX-2) indices.Results: PG significantly inhibited the uterine contraction of the primary dysmenorrhea rat model (p < 0.05), and was significantly different than single-agent therapy (p < 0.05). Histopathological examination showed inflammation in the uteri of the control group which YHP and its main constitutes alleviated. THP significantly inhibited the contraction of isolated uteri caused by Ach, PGF(2 alpha), and oxytocin in a concentration-dependent fashion. THP and IMP both significantly affected the levels of NO, activation of NF-kappa B, up-regulated the expression of i-kappa B and down-regulated the expression of both iNOS and COX-2. IMP obviously decreased the level of MDA and increased the activation of SOD (p < 0.05). PG obviously improved all the parameters mentioned above (p < 0.05).Conclusions: YHP exerted protective effects on primary dysmenorrhea in rats and remarkably alleviated the severity of experimental primary dysmenorrhea. The combined strategy proved to be more effective than either THP or IMP alone and may have synergistic effects in combination in primary dysmenorrhea. Mechanisms that might account for the beneficial effects include abating oxidative stress, inhibiting over-inflammatory reaction, and alleviating the contraction of isolated rat uteri by inhibiting the influx of extracellular Ca2+. Broad potential for future clinical practice is foreseeable. (C) 2013 Elsevier GmbH. All rights reserved.