THE SKELETAL AND CARDIAC ALPHA-ACTIN GENES ARE COEXPRESSED IN EARLY EMBRYONIC STRIATED-MUSCLE
THE SKELETAL AND CARDIAC ALPHA-ACTIN GENES ARE COEXPRESSED IN EARLY EMBRYONIC STRIATED-MUSCLE
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DOI：
10.1016/0012-1606(86)90315-5
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发表时间：
1986-10-01
影响因子：
2.7
通讯作者：
ORDAHL, CP
中科院分区：
文献类型：
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作者：
ORDAHL, CP
在胚胎骨骼肌和心肌(横纹肌)发育的不同阶段，心肌和骨骼肌α-肌动蛋白mRNAs的相对稳定丰度是通过使用与这两个mRNAs 5‘端附近的一个完全保守的区域互补的单一寡核苷酸引物的逆转录酶延伸试验来确定的。研究发现，这两种mRNAs都存在于胚胎横纹肌发育的每个阶段，包括肢体肌肉和心肌发育的最早可分析阶段。在骨骼肌发育的早期阶段，这两个mRNAs的水平相似，而在后期，骨骼肌α-肌动蛋白mRNA的丰度远远超过心脏α-肌动蛋白mRNA的丰度。在整个胚胎心肌发育过程中，这两种mRNAs的水平也是相似的，而在成年心肌中，心肌α-肌动蛋白信使核糖核酸比骨骼肌α-肌动蛋白信使核糖核酸占优势。这些关于早期胚胎横纹肌的结果，结合先前对胚胎晚期和成年横纹肌的研究结果，表明这两个基因在整个横纹肌个体发育过程中都是共表达的。因此，这两个基因可能不会在独特的组织特异性调控程序下进行调控，但每个基因都可能获得了调控元件，这些调控元件在成熟的横纹肌细胞中的表达水平具有重要的数量差异。
The relative steady-state abundance of cardiac and skeletal .alpha.-actin mRNAs at different stages of embryonic skeletal and cardiac (striated) muscle development was determined by a reverse transcriptase extension assay employing an single oligonucleotide primer complementary to a perfectly conserved region near the 5'' end of both mRNAs. Both mRNAs were found to be present at every stage of embryonic striated muscle development tested, including the earliest assayable stages of limb muscle and cardiac muscle development. At early stages of skeletal muscle development the two mRNAs are present at similar levels while at later stages the abundance of the skeletal .alpha.-actin mRNA far exceeds that of the cardiac .alpha.-actin mRNA. Both mRNAs are also present at similar levels throughout embryonic cardiac muscle development while in adult cardiac muscle the cardiac .alpha.-actin mRNA predominants over the skeletal .alpha.-actin mRNA. These results for early embryonic striated muscle, in combination with previous results with late embryonic and adult striated muscle, indicate that both genes are coexpressed throughout striated muscle ontogeny. These two genes may not, therefore, be regulated under unique tissue-specific regulatory programs but each may have acquired regulatory elements which confer important quantitative differences in their level of expression in mature striated muscle cells.