A unique cell division machinery in the Archaea
A unique cell division machinery in the Archaea
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DOI：
10.1073/pnas.0809467105
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发表时间：
2008-12-02
影响因子：
11.1
通讯作者：
Bernander, Rolf
中科院分区：
文献类型：
--
作者：
Lindas, Ann-Christin;Karlsson, Erik A.;Bernander, Rolf
与细菌、广古菌和真核生物的细胞分裂机制相反，在第二个主要古菌门——泉古菌门中尚未发现分裂成分。在这里，我们证明了嗜热硫化叶菌中的三基因操纵子 cdv 构成了独特的细胞分裂机制的一部分。操纵子在基因组分离和分裂开始时被诱导，然后 Cdv 蛋白在分离的核仁之间聚合，并在整个细胞分裂过程中持续存在，在收缩过程中形成逐渐变小的结构。 cdv 操纵子在紫外线照射后显着下调，表明对 DNA 损伤的反应受到分裂抑制，让人想起真核检查点系统。 cdv 基因相对于基于 FtsZ 的古菌分裂系统表现出互补的系统发育范围，使得在大多数古菌谱系中，存在一种或另一种系统。其中两种 Cdv 蛋白 CdvB 和 CdvC 与参与管腔囊泡出芽和 HIV-1 病毒粒子释放的真核 ESCRT-III 分选复合体的成分表现出同源性，表明机制相似和共同的进化起源。
In contrast to the cell division machineries of bacteria, euryarchaea, and eukaryotes, no division components have been identified in the second main archaeal phylum, Crenarchaeota. Here, we demonstrate that a three-gene operon, cdv, in the crenarchaeon Sulfolobus acidocaldarius, forms part of a unique cell division machinery. The operon is induced at the onset of genome segregation and division, and the Cdv proteins then polymerize between segregating nucleoids and persist throughout cell division, forming a successively smaller structure during constriction. The cdv operon is dramatically down-regulated after UV irradiation, indicating division inhibition in response to DNA damage, reminiscent of eukaryotic checkpoint systems. The cdv genes exhibit a complementary phylogenetic range relative to FtsZ-based archaeal division systems such that, in most archaeal lineages, either one or the other system is present. Two of the Cdv proteins, CdvB and CdvC, display homology to components of the eukaryotic ESCRT-III sorting complex involved in budding of luminal vesicles and HIV-1 virion release, suggesting mechanistic similarities and a common evolutionary origin.