A Xenopus homologue of aml-1 reveals unexpected patterning mechanisms leading to the formation of embryonic blood.
A Xenopus homologue of aml-1 reveals unexpected patterning mechanisms leading to the formation of embryonic blood.
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发表时间：
1998-04
期刊：
影响因子：
4.6
通讯作者：
W. D. Tracey;M. Pepling;Marko E. Horb;Gerald H. Thomsen;J. Gergen
中科院分区：
文献类型：
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作者：
W. D. Tracey;M. Pepling;Marko E. Horb;Gerald H. Thomsen;J. Gergen
Run结构域基因AML1在小鼠胚胎发育过程中对最终的造血起着至关重要的作用。我们分离了XAML，一种非洲爪哇AML1同源物，以研究导致造血祖细胞产生的模式机制。XAML在发育中的腹侧血岛早期以一种先于后来的珠蛋白的模式表达。对外植体、背腹型受扰胚胎中珠蛋白和XAML表达的分析以及谱系追踪表明，腹侧血岛的形成比之前认为的更复杂，涉及背侧和腹侧组织的贡献。截短的XAML蛋白会干扰原始造血。基于这些结果，我们认为Run结构域蛋白在脊椎动物胚胎中造血干细胞的指定中起作用。
The Runt domain gene AML1 is essential for definitive hematopoiesis during murine embryogenesis. We have isolated Xaml, a Xenopus AML1 homologue in order to investigate the patterning mechanisms responsible for the generation of hematopoietic precursors. Xaml is expressed early in the developing ventral blood island in a pattern that anticipates that of later globin. Analysis of globin and Xaml expression in explants, in embryos with perturbed dorsal ventral patterning, and by lineage tracing indicates that the formation of the ventral blood island is more complex than previously thought and involves contributions from both dorsal and ventral tissues. A truncated Xaml protein interferes with primitive hematopoiesis. Based on these results, we propose that Runt domain proteins function in the specification of hematopoietic stem cells in vertebrate embryos.