SIMPLE AND NOVEL METHOD FOR TRITIUM LABELING OF GANGLIOSIDES AND OTHER SPHINGOLIPIDS
SIMPLE AND NOVEL METHOD FOR TRITIUM LABELING OF GANGLIOSIDES AND OTHER SPHINGOLIPIDS
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DOI：
10.1016/0005-2760(78)90108-x
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发表时间：
1978-01-01
期刊：
影响因子：
--
通讯作者：
SCHWARZMANN, G
中科院分区：
文献类型：
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作者：
SCHWARZMANN, G
A very simple method for introducing 3H specifically into the ceramide portion of gangliosides, neutral glycosphingolipids and sphingomyelin was developed using potassium boro[3H]hydride and Pd as catalyst. In this way 6 different gangliosides, 5 different neutral glycosphingolipids and sphingomyelin [human brain] with high specific radioactivity were prepared for the first time. This simple procedure which does not require sophisticated apparatuses is suitable for large scale preparation of tritium-labeled sphingolipids and for nanogram quantities of individual sphingolipids so as to serve for analytical purposes. During the course of the labeling procedure no degradation of even the most labile trisialosyl-gangliotetraosylceramide was observed. The yield of tritiated compounds was almost quantitative. The specific radioactivity depended on the unsaturation of the ceramide moiety and the specific activity of the boro[3H]hydride employed. The labeled neutral glycosphingolipids were analyzed by autoradiography following TLC and by analyses of their hexose content. Gangliosides were analyzed by determination of their sialic acid content.
