Cytological and genetic analyses of a wheat-rye 2RL ditelosomic addition line with adult plant resistance to powdery mildew
Cytological and genetic analyses of a wheat-rye 2RL ditelosomic addition line with adult plant resistance to powdery mildew
复制标题
具有成年植物抗白粉病能力的小麦-黑麦 2RL 双端体附加系的细胞学和遗传分析
DOI：
10.1016/j.cj.2021.08.011
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发表时间：
2022-06-01
期刊：
影响因子：
6.6
通讯作者：
Zhang, Xiaotian
中科院分区：
文献类型：
--
作者：
An, Diaoguo;Han, Guohao;Zhang, Xiaotian
黑麦（Secale cereale genome RR）是小麦的近缘种，具有重要的抗病基因。由于病原菌毒力和寄主抗性的协同进化，黑麦中的一些抗性基因已经失效。因此，需要从黑麦中开发和鉴定新的、有效的抗性基因。本研究通过远缘杂交、胚挽救培养、染色体加倍和回交获得了小麦-黑麦新品系WR 56。通过基因组原位杂交（genomic in situ hybridization，GISH）、荧光原位杂交（mc-FISH）、非变性荧光原位杂交（ND-FISH）、多色荧光原位杂交（multicolor GISH）和黑麦染色体臂特异性标记分析，证明WR 56是小麦-黑麦2 RL双端体附加系。WR 56对小麦白粉病表现出高水平的成株抗性。（Bgt.）这种抗性是由附加的2 RL端体携带的，推测它与同样位于2 RL染色体臂上但在苗期和成株期具有抗性的Pm 7不同。WR 56是一个很有前途的桥梁亲本，可将抗病性转移到更稳定的小麦育种系中。新开发的2 RL特异性KASP（竞争等位基因特异性PCR）标记应该加快这项工作。(c)2021中国作物学会、中国农业科学院作物科学研究所。Elsevier B. V.代表KeAi Communications Co.制作和主持，这是一个在CC BY-NC -ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
Rye (Secale cereale genome RR), a close relative of common wheat, possesses valuable resistance genes for wheat improvement. Due to the co-evolution of pathogen virulence and host resistance, some resistance genes derived from rye have lost effectiveness. Development and identification of new, effective resis-tance genes from rye is thus required. In the current study, wheat-rye line WR56 was produced through distant hybridization, embryo rescue culture, chromosome doubling and backcrossing. WR56 was then proved to be a wheat-rye 2RL ditelosomic addition line using GISH (genomic in situ hybridization), mc-FISH (multicolor fluorescence in situ hybridization), ND-FISH (non-denaturing FISH), mc-GISH (mul-ticolor GISH) and rye chromosome arm-specific marker analysis. WR56 exhibited a high level of adult plant resistance to powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). This resistance was car-ried by the added 2RL telosomes and presumed to be different from Pm7 which is also located on chro-mosome arm 2RL but confers resistance at the seedling and adult stages. WR56 will be a promising bridging parent for transfer of the resistance to a more stable wheat breeding line. A newly developed 2RL-specific KASP (kompetitive allele specific PCR) marker should expedite that work.(c) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).