Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity
Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity
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含 bFGF 的支架中增强的血管生成促进了封闭肝细胞的活力并维持了肝特异性糖原储存能力
DOI：
10.1252/jcej.38.913
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发表时间：
2005
影响因子：
0.8
通讯作者：
K. Kawakami
中科院分区：
文献类型：
--
作者：
T. Takei;H. Ijima;S. Sakai;T. Ono;K. Kawakami
我们制作了一个聚乳酸和酸性明胶支架，碱性成纤维细胞生长因子（bFGF）的缓释载体，是血管生成的积极调节因子。植入大鼠肠系膜1周后，含bfgf支架诱导的血管数量几乎是不含bfgf支架的两倍。此外，在接受70%肝切除术的大鼠肠系膜内植入1周后的肝细胞固定化支架的组织学检查显示，含bfgf的支架比不含bfgf的支架更有效地固定细胞存活。同时，含bfgf支架中的肝细胞也保持了肝特异性糖原储存能力。
We fabricated a scaffold of poly (lactic acid) and acidic gelatin, a sustained-release carrier of basic fibroblast growth factor (bFGF), which is a positive regulator of angiogenesis. After 1 week of implantation into mesentery of rats, blood vessels induced in the bFGF-containing scaffolds were almost double those in the bFGF-free scaffolds. Further, histological examination of hepatocyte-immobilizing scaffolds retrieved after 1 week of implantation into the mesentery of the rats that received a 70% hepatectomy revealed that the bFGF-containing scaffolds were more efficient for immobilized cell survival than the bFGF-free scaffolds. As well, hepatocytes in the bFGF-containing scaffolds kept their hepatospecific glycogen storage capacity.