African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site
African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site
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DOI：
10.1099/vir.0.025874-0
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发表时间：
2011-02-01
影响因子：
3.8
通讯作者：
Bishop, Richard P.
中科院分区：
文献类型：
--
作者：
Gallardo, Carmina;Okoth, Edward;Bishop, Richard P.
非洲猪瘟病毒（ASFV）的祖先森林循环的作用在非洲东部的流行地区还没有得到很好的理解。因此，我们分析了ASF感染状况的样本收集自51个自由放养的疣猪（Phacocherus africanus）和1576 Omithodorus porcinus蜱从26个独立的疣猪洞穴在肯尼亚的一个牧场。本研究纳入了来自83头无临床症状家猪的屠宰场样本，这些家猪来自最近未报告ASF爆发的特定地点。所有样本均来自肯尼亚中部地区，过去曾报告过ASF疫情。22%的O.基于p72的PCR检测结果，对牛肝菌池、成年疣猪血清样本和家猪血清样本的检测率分别为3.22%和49%。用ELISA法检测，所有野猪血清均为抗ASFV抗体阳性，而家猪血清均为阴性。二十在4个多态性位点对猪源病毒、12个家猪源病毒和3个疣猪源病毒进行了基因分型。从蜱和家猪中分离的ASFV聚集在p72基因型X内。相比之下，在与蜱分离株相同的地点，直接从疣猪血清中基因分型的ASF病毒在p72基因型IX内，并且与最近在肯尼亚和乌干达引起ASF暴发的病毒在遗传上相似。这代表了不同的ASFV基因型共存的第一份报告，在疣猪洞穴相关的蜱和成年野生疣猪。这项研究和早期研究的数据表明，在东非，至少有两种不同p72基因型的病毒从野猪转移到家猪。
The role of the ancestral sylvatic cycle of the African swine fever virus (ASFV) is not well understood in the endemic areas of eastern Africa. We therefore analysed the ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Omithodorus porcinus ticks from 26 independent warthog burrows at a single ranch in Kenya. Abattoir samples from 83 domestic pigs without clinical symptoms, originating from specific locations with no recent reported ASF outbreaks were included in this study. All samples were derived from areas of central Kenya, where ASF outbreaks have been reported in the past. Infection with ASFV was confirmed in 22% of O. porcinus pools, 3.22% of adult warthog serum samples and 49% of domestic pig serum samples by using p72-based PCR. All of the warthog sera were positive for anti-ASFV antibodies, investigated by using ELISA, but none of the domestic pig sera were positive. Twenty 0. porcinus-, 12 domestic pig- and three warthog-derived viruses were genotyped at four polymorphic loci. The ASFV isolates from ticks and domestic pigs clustered within p72 genotype X. By contrast, ASF viruses genotyped directly from warthog sera, at same locality as the tick isolates, were within p72 genotype IX and genetically similar to viruses causing recent ASF outbreaks in Kenya and Uganda. This represents the first report of the co-existence of different ASFV genotypes in warthog burrow-associated ticks and adult wild warthogs. The data from this and earlier studies suggest transfer of viruses of at least two different p72 genotypes, from wild to domestic pigs in East Africa.