Expression profiles of microRNAs encoded by the oncogenic Marek's disease virus reveal two distinct expression patterns in vivo during different phases of disease
Expression profiles of microRNAs encoded by the oncogenic Marek's disease virus reveal two distinct expression patterns in vivo during different phases of disease
复制标题
致癌马立克氏病病毒编码的 microRNA 表达谱揭示了疾病不同阶段体内两种不同的表达模式
DOI：
10.1099/vir.0.024158-0
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发表时间：
2011-03-01
影响因子：
3.8
通讯作者：
Zhang, Gai-Ping
中科院分区：
文献类型：
--
作者：
Luo, Jun;Sun, Ai-Jun;Zhang, Gai-Ping
马立克氏病病毒(MDV)是一种公认的致癌性疱疹病毒，可在其自然宿主中诱发淋巴瘤，可通过接种疫苗预防。MDV感染为研究病毒致癌的生物学、遗传学和免疫学提供了一个很好的生物学模型。最近在MDV基因组中发现的microRNAs(MiRNAs)被认为在MDV的肿瘤发生过程中具有调节作用。我们检测了以前报道的所有22个由MDV-1编码的miRNAs在MDV-GX0101人工攻击的鸡体内的表达谱。我们发现，在疾病发展的不同阶段，miRNAs的一部分存在差异表达。这些miRNAs在疾病进展过程中表现出早期或晚期的表达，并伴随着明显的组织特异性和差异表达模式。这种时间性和差异性的组织分布表明，这些miRNAs可能在从潜伏期转换到溶血性复制、免疫抑制、肿瘤转化或淋巴瘤形成的其他方面发挥不同的调节作用。这些报道的体内表达谱表明，MDV-1编码的潜在功能miRNAs应该被选择来进一步研究它们在MDV肿瘤发生中的功能。
Marek's disease virus (MDV) is a long-recognized oncogenic herpesvirus, which induces lymphoma in its natural host that can be prevented by vaccination. MDV infection provides an excellent biological model for investigating the biology, genetics and immunology of viral oncogenesis. Recently discovered microRNAs (miRNAs) in the MDV genome have been suggested to have regulatory roles during MDV oncogenesis. We have examined the expression profiles of all 22 previously reported miRNAs encoded by MDV-1 in chickens artificially challenged with MDV-GX0101. We found that a subset of the miRNAs was differentially expressed during different phases of the developing disease. These miRNAs show early or late expression during disease progression, accompanied by obvious tissue-specific and differential expression patterns. This temporal and differential tissue distribution suggest that these miRNAs may perform different regulatory roles in switching from latency to lytic replication, immunosupression, neoplastic transformation or other aspects of lymphoma formation. These reported in vivo expression profiles indicate the potentially functional MDV-1-encoded miRNAs that should be selected for further investigation of their functions in MDV oncogenesis.