Carbon catabolite repression relaxation in Escherichia coli: global and sugar-specific methods for glucose and secondary sugar co-utilization
Carbon catabolite repression relaxation in Escherichia coli: global and sugar-specific methods for glucose and secondary sugar co-utilization
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DOI：
10.1016/j.coche.2020.05.005
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发表时间：
2020-12-01
影响因子：
6.6
通讯作者：
Prather, Kristala L. J.
中科院分区：
文献类型：
--
作者：
Fox, Kevin J.;Prather, Kristala L. J.
利用农业废弃物的能力是使用大肠杆菌进行生物处理成为不可持续的基于化石燃料的化学合成方法的有吸引力的替代方案的众多原因之一。然而，由于碳分解代谢物抑制（CCR）的影响，混合糖的共同利用对大肠杆菌来说是一个挑战，这决定了通过诱导物排除和转录调节机制优先消耗葡萄糖而不是次级糖。为了克服这些障碍，代谢工程师制定了通过全局方法和糖特异性方法来缓解 CCR 影响的策略。了解饲料条件以及 CCR 松弛的工程机会可以允许开发具有最大生产力和生长的菌株。
The ability to utilize agricultural waste products is one of the many reasons why bioprocessing using Escherichia coli is an attractive alternative to unsustainable fossil fuel-based methods for chemical synthesis. However, mixed sugar co utilization is a challenge for E. coli due to the effects of carbon catabolite repression (CCR), which dictates a preference for the consumption of glucose over secondary sugars through the mechanisms of inducer exclusion and transcriptional regulation. To overcome these obstacles, metabolic engineers have developed strategies to relax the effects of CCR through both global and sugar-specific methods. Understanding the feed conditions as well as the engineering opportunities for CCR relaxation can allow for the development of a strain with maximized productivity and growth.