X-ray fluorescence microscopy of zinc localization in wheat grains biofortified through foliar zinc applications at different growth stages under field conditions
X-ray fluorescence microscopy of zinc localization in wheat grains biofortified through foliar zinc applications at different growth stages under field conditions
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DOI：
10.1007/s11104-015-2467-8
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发表时间：
2015-07-01
期刊：
影响因子：
4.9
通讯作者：
McLaughlin, Michael J.
中科院分区：
文献类型：
--
作者：
Ajiboye, Babasola;Cakmak, Ismail;McLaughlin, Michael J.
通过叶面施锌对小麦进行生物锌强化是提高小麦籽粒锌含量的一种农艺策略，可作为缺锌地区的一种营养干预措施。在不同生长阶段通过叶面施锌进行生物强化。用电感耦合等离子体发射光谱法测定了收获的全谷物中锌和相关微量营养素的浓度。基于同步辐射的X射线荧光显微镜（XFM），然后被用来调查这些grain.The浓度的锌和其他微量元素（锰，铁，铜）的横截面中的锌和相关的微量元素的本地化处理的谷物在籽粒灌浆（早期牛奶/面团）比那些处理在茎伸长。小麦籽粒灌浆期叶面喷施Zn浓度的增加是由于Zn在糊粉层、改良糊粉层、折痕组织、维管束和胚乳腔中的强烈定位，而在籽粒最主要的组织胚乳中的定位较弱。这些组织和它们所含的锌被认为是保留在研磨后，可以潜在地增加锌浓度在小麦flourish.By使用XFM，它表明，叶面喷锌是一个重要的农艺工具，大量锌富集的小麦籽粒的不同部分，特别是胚乳。建议进一步调查胚乳中锌的化学形态，以评估锌的生物利用度在收获的全粒小麦，已通过不同的时间叶面施锌生物强化。
Biofortification of wheat with zinc (Zn) through foliar Zn application has been proposed as an agronomic strategy to increase grain Zn concentration, which could serve as a nutritional intervention in regions with dietary Zn deficiency.Bread wheat (Triticum aestivum L.) was biofortified through foliar Zn applications at different growth stages. The concentration of Zn and associated micronutrient in harvested whole grains was determined by ICP-OES. Synchrotron-based X-ray fluorescence microscopy (XFM) was then used to investigate the localization of Zn and associated micronutrients in cross sections of these grains.The concentration of Zn and other micronutrients (Mn, Fe, and Cu) was higher in grains treated with foliar Zn during grain-filling (early milk/dough) than those treated at stem elongation. The increase in Zn concentration of wheat grain with foliar application during grain-filling can be attributed to the intense localization of Zn in the aleurone layer, modified aleurone, crease tissue, vascular bundle, and endosperm cavity, and to a modest localization in endosperm, which is the most dominant grain tissue. These tissues and the Zn they contain are presumed to remain after milling and can potentially increase the Zn concentration in wheat flour.By using XFM, it was shown that foliar Zn spray represents an important agronomic tool for a substantial Zn enrichment of different fractions of wheat grain, especially the endosperm. Further investigation of the chemical speciation of Zn in the endosperm is recommended to assess Zn bioavailability in harvested whole grain of wheat that has been biofortified through different timing of foliar Zn application.